Казанский (Приволжский) федеральный университет, КФУ
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THE TEMPERATURE-DEPENDENT SELECTIVITY OF POTENTIAL INTERACTION PARTNERS FOR THE SMALL HEAT SHOCK PROTEIN IBPA FROM ACHOLEPLASMA LAIDLAWII
Форма представленияСтатьи в зарубежных журналах и сборниках
Год публикации2016
Языканглийский
  • Каюмов Айрат Рашитович, автор
  • Богачев Михаил Игоревич, автор
  • Борщеус Сергей , автор
  • Вишняков Иннокентий , автор
  • Салафутдинов Ильнур Ильдусович, автор
  • Библиографическое описание на языке оригинала Vishnyakov I.E. The Temperature-Dependent Selectivity of Potential Interaction Partners for the Small Heat Shock Protein IbpA from Acholeplasma laidlawii /I.E. Vishnyakov, M. Bogachev, I. Salafutdinov, S.N. Borchsenius, A.R. Kayumov // BioNanoScience. - 2016. DOI: 10.1007/s12668-016-0259-0.
    Аннотация Small heat shock proteins (sHsps) of α-crystalline type play a key role in the cell survival under stress conditions by preventing irreversible denaturation and aggregation of proteins. In contrast to most Mollicutes (mycoplasmas) where no sHsps were found, recently, some sHsp homologs were identified in the Acholeplasmataceae family, including Acholeplasma laidlawii, the only representative of Mollicutes that is known to survive in a host-free environment. Using pull-down followed by LC-MS, we identified the potential target proteins co-eluting with IbpA from the A. laidlawii cell extracts after exposition to low- and high temperatures. 308 and 464 proteins were co-eluted with IbpA from the cold- and heat-treated extracts, respectively, while only 240 of them were co-eluted with sHsp independently of the temperature. Most of potential IbpA targets were identified as enzymes involved in biosynthetic cycles and energy metabolism. We show IbpA specificity for target proteins on the incub
    Ключевые слова Acholeplasma laidlawii, Small heat shockproteins (sHsps), IbpA, Heat shock, Cold shock
    Название журнала BioNanoScience
    URL https://www.researchgate.net/publication/307561121_The_Temperature-Dependent_Selectivity_of_Potential_Interaction_Partners_for_the_Small_Heat_Shock_Protein_IbpA_from_Acholeplasma_laidlawii
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