КФУ. Карточка публикации. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuriesa

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HTRA PROTEASE FROM BACILLUS SUBTILIS SUPPRESSES THE BACTERIAL FOULING OF THE RAT SKIN INJURIESA

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2016

Язык

английский

Авторы, сотрудники КФУ

Ахметов Нафис Фанисович, автор

Каюмов Айрат Рашитович, автор

Другие авторы

Богачев Михаил Игоревич, автор

Авторы, аспиранты КФУ

Балтин Максим Эдуардович, автор

Шарафутдинов Иршад Султанович, автор

Авторы, студенты КФУ

Шигапова Зухра Рашидовна, автор

Библиографическое описание на языке оригинала

Sharafutdinov I. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuries / I. Sharafutdinov, Z. Shigapova, M. Baltin, N. Akhmetov, M. Bogachev, A. Kayumov // BioNanoScience. – 2016 – V.6(4). – P.564-567.

Аннотация

The gene of serine protease HtrA from Bacillus subtilis was cloned and recombinant protein was overexpressed in E.coli and purified. The recombinant HtrA efficiently repressed in vitro the biofilm formation by clinical isolates of Staphylococcus aureus. While the model rat skin injuries treated with HtrA healed slower than in the case of chymotrypsin, their recovery was significantly faster in compare with pure storage buffer. On the other hand, the number of bacterial CFUs on the injuries treated with HtrA solution was reduced five times, similarly to chymotrypsin treated ones. By the way, the resident microflora content of protease-treated and control wounds remained almost similar within 4 days, with Enterococcus faecalis and Staphylococcus epidermidis being the main resident microflora after the treatment. To the 8th day, the amount of staphylococcal cells was drastically reduced on HtrA- and chymotrypsin treated wound surfaces, confirming that both proteases provide wound cleaning

Ключевые слова

Serine proteases, Bacillus subtilis, HtrA

Название журнала

BioNanoScience

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https://repository.kpfu.ru/?p_id=138243

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ахметов Нафис Фанисович	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Богачев Михаил Игоревич	ru_RU
dc.contributor.author	Шигапова Зухра Рашидовна	ru_RU
dc.contributor.author	Балтин Максим Эдуардович	ru_RU
dc.contributor.author	Шарафутдинов Иршад Султанович	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Sharafutdinov I. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuries / I. Sharafutdinov, Z. Shigapova, M. Baltin, N. Akhmetov, M. Bogachev, A. Kayumov // BioNanoScience. – 2016 – V.6(4). – P.564-567.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=138243	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	The gene of serine protease HtrA from Bacillus subtilis was cloned and recombinant protein was overexpressed in E.coli and purified. The recombinant HtrA efficiently repressed in vitro the biofilm formation by clinical isolates of Staphylococcus aureus. While the model rat skin injuries treated with HtrA healed slower than in the case of chymotrypsin, their recovery was significantly faster in compare with pure storage buffer. On the other hand, the number of bacterial CFUs on the injuries treated with HtrA solution was reduced five times, similarly to chymotrypsin treated ones. By the way, the resident microflora content of protease-treated and control wounds remained almost similar within 4 days, with Enterococcus faecalis and Staphylococcus epidermidis being the main resident microflora after the treatment. To the 8th day, the amount of staphylococcal cells was drastically reduced on HtrA- and chymotrypsin treated wound surfaces, confirming that both proteases provide wound cleaning	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Serine proteases	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	HtrA	ru_RU
dc.title	HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuriesa	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU