КФУ. Карточка публикации. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red

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LABEL-FREE ELECTROCHEMICAL APTASENSOR FOR CYTOCHROME C DETECTION USING PILLAR[5]ARENE BEARING NEUTRAL RED

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2016

Язык

английский

Авторы, сотрудники КФУ

Евтюгин Владимир Геннадьевич, автор

Евтюгин Геннадий Артурович, автор

Осин Юрий Николаевич, автор

Стойков Иван Иванович, автор

Шурпик Дмитрий Николаевич, автор

Библиографическое описание на языке оригинала

Stepanova, V.B. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red [Text] / V.B.Stepanova, D.N.Shurpik, V.G.Evtugyn, I.I.Stoikov, G.A.Evtugyn, Yu.N.Osin, T.Hianik // Sens. Actuators B.- 2016. - V.225. - P.57-65.

Аннотация

Novel electrochemical aptasensor toward cytochrome c (Cyt c) has been developed on the base of glassy carbon electrode (GCE) modified with electropolymerized neutral red (Poly-NR) and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red (NR) and aminated aptamer specific to Cyt c. Addition of Cyt c resulted in decrease of the cathodic peak current of NR on cyclic voltammogram due to suppression of the electron exchange between reduced and oxidized NR forms in the surface layer. The implementation of Cyt c in the surface layer was confirmed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Depending on the content of the surface layer and assembling protocol, the limits of detection (LODs) varied from 0.02 to 1.0 nM and linear range of concentrations was within three orders of magnitude. Interfering influence of some proteins and polyethylene glycol was characterized.

Ключевые слова

DNA aptamer, cytochrome c, electrochemical sensor, poly(neutral red), pillar[5]arene

Название журнала

Sensors and Actuators, B: Chemical

URL

http://www.scopus.com/inward/record.url?eid=2-s2.0-84947575990&partnerID=40&md5=f9cb7437ad86907d0cc1152af33b1c9f

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https://repository.kpfu.ru/?p_id=121478

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Евтюгин Владимир Геннадьевич	ru_RU
dc.contributor.author	Евтюгин Геннадий Артурович	ru_RU
dc.contributor.author	Осин Юрий Николаевич	ru_RU
dc.contributor.author	Стойков Иван Иванович	ru_RU
dc.contributor.author	Шурпик Дмитрий Николаевич	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Stepanova, V.B. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red [Text] / V.B.Stepanova, D.N.Shurpik, V.G.Evtugyn, I.I.Stoikov, G.A.Evtugyn, Yu.N.Osin, T.Hianik // Sens. Actuators B.- 2016. - V.225. - P.57-65.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=121478	ru_RU
dc.description.abstract	Sensors and Actuators, B: Chemical	ru_RU
dc.description.abstract	Novel electrochemical aptasensor toward cytochrome c (Cyt c) has been developed on the base of glassy carbon electrode (GCE) modified with electropolymerized neutral red (Poly-NR) and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red (NR) and aminated aptamer specific to Cyt c. Addition of Cyt c resulted in decrease of the cathodic peak current of NR on cyclic voltammogram due to suppression of the electron exchange between reduced and oxidized NR forms in the surface layer. The implementation of Cyt c in the surface layer was confirmed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Depending on the content of the surface layer and assembling protocol, the limits of detection (LODs) varied from 0.02 to 1.0 nM and linear range of concentrations was within three orders of magnitude. Interfering influence of some proteins and polyethylene glycol was characterized.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	DNA aptamer	ru_RU
dc.subject	cytochrome c	ru_RU
dc.subject	electrochemical sensor	ru_RU
dc.subject	poly(neutral red)	ru_RU
dc.subject	pillar[5]arene	ru_RU
dc.title	Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU