КФУ. Карточка публикации. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants

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EXPRESSION OF PANTOEA AGGLOMERANS PHYTASE FROM A STRONG CONSTITUTIVE PROMOTER IN ARABIDOPSIS THALIANA PLANTS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2015

Язык

русский

Авторы, сотрудники КФУ

Бульмакова Дарья Сергеевна, автор

Валеева Лия Рашитовна, автор

Нямсурэн Чулуунцэцэг ., автор

Шакиров Евгений Витальевич, автор

Шарипова Маргарита Рашидовна, автор

Авторы, аспиранты КФУ

Валеева Лия Рашитовна, автор

Библиографическое описание на языке оригинала

Valeeva, L. R. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants / L. R Valeeva, Ch. Nyamsuren, D. S Troshagina, M. R Sharipova, E. V. Shakirov // RJPBCS. - 2015. - V. 6(4). - P.99-104.

Аннотация

In this study we report construction of an efficient gene expression system in plants and subsequent characterization of transgenic Arabidopsis thaliana expressing a bacterial paPhyC phytase gene from Pantoea sp. Phytase gene expression is controlled by a strong 35S constitutive promoter from cauliflower mosaic virus. All identified transgenic plants had multiple T-DNA insertions in the genome. Expression of paPhyC phytase mRNA in plant tissue was confirmed by RT-PCR in the second generation of transgenic plants, and phytase protein expression was confirmed by Western blotting. Our data indicate that bacterial phytase expression in plants can be an efficient way to potentially increase crop performance in conditions of inorganic phosphorus deficiency in the soil.

Ключевые слова

phytase Pantoea sp., recombinant protein expression in plants, CaMV35S promoter, transgenic plants, phosphorus deficiency, Arabidopsis thaliana

Название журнала

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6(4)/[17].pdf

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https://repository.kpfu.ru/?p_id=125195

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Бульмакова Дарья Сергеевна	ru_RU
dc.contributor.author	Валеева Лия Рашитовна	ru_RU
dc.contributor.author	Нямсурэн Чулуунцэцэг .	ru_RU
dc.contributor.author	Шакиров Евгений Витальевич	ru_RU
dc.contributor.author	Шарипова Маргарита Рашидовна	ru_RU
dc.contributor.author	Валеева Лия Рашитовна	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Valeeva, L. R. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants / L. R Valeeva, Ch. Nyamsuren, D. S Troshagina, M. R Sharipova, E. V. Shakirov // RJPBCS. - 2015. - V. 6(4). - P.99-104.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=125195	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	In this study we report construction of an efficient gene expression system in plants and subsequent characterization of transgenic Arabidopsis thaliana expressing a bacterial paPhyC phytase gene from Pantoea sp. Phytase gene expression is controlled by a strong 35S constitutive promoter from cauliflower mosaic virus. All identified transgenic plants had multiple T-DNA insertions in the genome. Expression of paPhyC phytase mRNA in plant tissue was confirmed by RT-PCR in the second generation of transgenic plants, and phytase protein expression was confirmed by Western blotting. Our data indicate that bacterial phytase expression in plants can be an efficient way to potentially increase crop performance in conditions of inorganic phosphorus deficiency in the soil.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	phytase Pantoea sp.	ru_RU
dc.subject	recombinant protein expression in plants	ru_RU
dc.subject	CaMV35S promoter	ru_RU
dc.subject	transgenic plants	ru_RU
dc.subject	phosphorus deficiency	ru_RU
dc.subject	Arabidopsis thaliana	ru_RU
dc.title	Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU