| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Хазипов Рустем Нариманович, автор
|
|
Baude Agnes , автор
Tressard Thomas , автор
|
| Библиографическое описание на языке оригинала |
Valeeva G. An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons / G. Valeeva, Т. Tressard, М. Mukhtarov, А. Baude, R. Khazipov // J Neurosci. - Vol. 36. - № 22. - Р. 5961-5973. |
| Аннотация |
To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a
mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings
from hippocampal and neocortical slices from postnatal day (P) 2?P15 mice, photostimulation caused depolarization and excitation
of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal cells were
assessed by monitoring the alteration in the frequency of EPSCs during photostimulation of interneurons. We found that in slices from
P2?P8 mice, photostimulation evoked an increase in EPSC frequency, whereas in P9 ?P15 mice the response switched to a reduction in
EPSC frequency, indicating a developmental excitatory-to-inhibitory switch in GABA actions on glutamatergic neurons. Using a similar
approach in urethane-anesthetized animals in vivo, we found that phot |
| Ключевые слова |
ChR2, cortex, GABA, hippocampus, inhibition, interneurons, patch clamp, optogenetics |
| Название журнала |
J NEUROSCI
|
| URL |
http://www.jneurosci.org/content/36/22/5961.long |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=135715 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Хазипов Рустем Нариманович |
ru_RU |
| dc.contributor.author |
Baude Agnes |
ru_RU |
| dc.contributor.author |
Tressard Thomas |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Valeeva G. An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons / G. Valeeva, Т. Tressard, М. Mukhtarov, А. Baude, R. Khazipov // J Neurosci. - Vol. 36. - № 22. - Р. 5961-5973. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=135715 |
ru_RU |
| dc.description.abstract |
J NEUROSCI |
ru_RU |
| dc.description.abstract |
To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a
mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings
from hippocampal and neocortical slices from postnatal day (P) 2?P15 mice, photostimulation caused depolarization and excitation
of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal cells were
assessed by monitoring the alteration in the frequency of EPSCs during photostimulation of interneurons. We found that in slices from
P2?P8 mice, photostimulation evoked an increase in EPSC frequency, whereas in P9 ?P15 mice the response switched to a reduction in
EPSC frequency, indicating a developmental excitatory-to-inhibitory switch in GABA actions on glutamatergic neurons. Using a similar
approach in urethane-anesthetized animals in vivo, we found that phot |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
ChR2 |
ru_RU |
| dc.subject |
cortex |
ru_RU |
| dc.subject |
GABA |
ru_RU |
| dc.subject |
hippocampus |
ru_RU |
| dc.subject |
inhibition |
ru_RU |
| dc.subject |
interneurons |
ru_RU |
| dc.subject |
patch clamp |
ru_RU |
| dc.subject |
optogenetics |
ru_RU |
| dc.title |
Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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