| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Гаранина Екатерина Евгеньевна, автор
Гумерова Аниса Азатовна, автор
Заикина Эльвира Ильдаровна, автор
Киясов Андрей Павлович, автор
Мавликеев Михаил Олегович, автор
Ризванов Альберт Анатольевич, автор
Сахапов Дамир Ильгизович, автор
Титова Ангелина Андреевна, автор
Шафигуллина Айгуль Касыймовна, автор
|
|
Мавликеев Михаил Олегович, автор
Титова Ангелина Андреевна, автор
|
| Библиографическое описание на языке оригинала |
Shafigullina, A. K. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype / A.K. Shafigullina, E.I. Zaikina, E.E. Garanina, D.I. Sakhapov, A.A. Titova, M.O. Mavlikeev, A.A. Rizvanov, A.A. Gumerova, A.A. Kiassov // BioNanoScience. - 2016. - Vol. 6. - Р. 1-4. |
| Аннотация |
Adenovirus is a promising gene delivery vector that has a high efficiency and relative ease of construction. These advantages make this system attractive for diverse research applications. In this project we performed transduction of hepatics stellate cells (HSC) with adenoviral vector containing hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene didn`t change their morphology, but stimulated expression of HSC marker desmin, lead to HSC activation (α-SMA) and appearance of α-FP, one of the hepatoblasts markers. During the entire ex |
| Ключевые слова |
hepatic stellate cells, adenovirus, transduction, differentiation |
| Название журнала |
BioNanoScience
|
| Ссылка для РПД |
http://dspace.kpfu.ru/xmlui/bitstream/handle/net/108481/Bionanoscience_Aygul_Phenotype_of_HSC.pdf?sequence=1&isAllowed=y
|
| URL |
http://link.springer.com/article/10.1007/s12668-016-0378-7 |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=147227 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Гаранина Екатерина Евгеньевна |
ru_RU |
| dc.contributor.author |
Гумерова Аниса Азатовна |
ru_RU |
| dc.contributor.author |
Заикина Эльвира Ильдаровна |
ru_RU |
| dc.contributor.author |
Киясов Андрей Павлович |
ru_RU |
| dc.contributor.author |
Мавликеев Михаил Олегович |
ru_RU |
| dc.contributor.author |
Ризванов Альберт Анатольевич |
ru_RU |
| dc.contributor.author |
Сахапов Дамир Ильгизович |
ru_RU |
| dc.contributor.author |
Титова Ангелина Андреевна |
ru_RU |
| dc.contributor.author |
Шафигуллина Айгуль Касыймовна |
ru_RU |
| dc.contributor.author |
Мавликеев Михаил Олегович |
ru_RU |
| dc.contributor.author |
Титова Ангелина Андреевна |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Shafigullina, A. K. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype / A.K. Shafigullina, E.I. Zaikina, E.E. Garanina, D.I. Sakhapov, A.A. Titova, M.O. Mavlikeev, A.A. Rizvanov, A.A. Gumerova, A.A. Kiassov // BioNanoScience. - 2016. - Vol. 6. - Р. 1-4. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=147227 |
ru_RU |
| dc.description.abstract |
BioNanoScience |
ru_RU |
| dc.description.abstract |
Adenovirus is a promising gene delivery vector that has a high efficiency and relative ease of construction. These advantages make this system attractive for diverse research applications. In this project we performed transduction of hepatics stellate cells (HSC) with adenoviral vector containing hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene didn`t change their morphology, but stimulated expression of HSC marker desmin, lead to HSC activation (α-SMA) and appearance of α-FP, one of the hepatoblasts markers. During the entire ex |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
hepatic stellate cells |
ru_RU |
| dc.subject |
adenovirus |
ru_RU |
| dc.subject |
transduction |
ru_RU |
| dc.subject |
differentiation |
ru_RU |
| dc.title |
Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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