КФУ. Карточка публикации. Targeting microbial biofilms using Ficin, a nonspecific plant protease

КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

RUS

- EN
- 中文
- ES

TARGETING MICROBIAL BIOFILMS USING FICIN, A NONSPECIFIC PLANT PROTEASE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2017

Язык

английский

Авторы, сотрудники КФУ

Ахатова Фарида Сериковна, автор

Байдамшина Диана Рафисовна, автор

Богачев Михаил Игоревич, автор

Каюмов Айрат Рашитович, автор

Рожина Эльвира Вячеславовна, автор

Тризна Елена Юрьевна, автор

Фахруллин Равиль Фаридович, автор

Другие авторы

Artyukhov V. G., автор

Holyavka M. G., автор

Библиографическое описание на языке оригинала

Baidamshina D.R. Targeting microbial biofilms using Ficin, a nonspecific plant protease / D.R. Baidamshina, E.Y. Trizna, M.G. Holyavka, M.I. Bogachev, V.G. Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Scientific Reports. - 2017. - V.7. - Art. № 46068.

Аннотация

Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 μg/ml and six-fold at 1000 μg/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via di

Ключевые слова

microbial biofilms, AFM, confocal microscopy, antibacterial, ficin

Название журнала

SCIENTIFIC REPORTS

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017260158&doi=10.1038%2fsrep46068&partnerID=40&md5=6d76c9287d36df3d056c487505a87d33

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=156993

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ахатова Фарида Сериковна	ru_RU
dc.contributor.author	Байдамшина Диана Рафисовна	ru_RU
dc.contributor.author	Богачев Михаил Игоревич	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Рожина Эльвира Вячеславовна	ru_RU
dc.contributor.author	Тризна Елена Юрьевна	ru_RU
dc.contributor.author	Фахруллин Равиль Фаридович	ru_RU
dc.contributor.author	Artyukhov V. G.	ru_RU
dc.contributor.author	Holyavka M. G.	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Baidamshina D.R. Targeting microbial biofilms using Ficin, a nonspecific plant protease / D.R. Baidamshina, E.Y. Trizna, M.G. Holyavka, M.I. Bogachev, V.G. Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Scientific Reports. - 2017. - V.7. - Art. № 46068.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=156993	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 μg/ml and six-fold at 1000 μg/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via di	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	microbial biofilms	ru_RU
dc.subject	AFM	ru_RU
dc.subject	confocal microscopy	ru_RU
dc.subject	antibacterial	ru_RU
dc.subject	ficin	ru_RU
dc.title	Targeting microbial biofilms using Ficin, a nonspecific plant protease	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU