КФУ. Карточка публикации. Targeting microbial biofilms using ficin, a nonspecific plant protease

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TARGETING MICROBIAL BIOFILMS USING FICIN, A NONSPECIFIC PLANT PROTEASE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2017

Авторы, сотрудники КФУ

Ахатова Фарида Сериковна, автор

Байдамшина Диана Рафисовна, автор

Богачев Михаил Игоревич, автор

Рожина Эльвира Вячеславовна, автор

Тризна Елена Юрьевна, автор

Фахруллин Равиль Фаридович, автор

Другие авторы

Холявка Марина Геннадьевна, Артюхов Валерий Григорьевич

Авторы, аспиранты КФУ

Тризна Елена Юрьевна, автор

Библиографическое описание на языке оригинала

Baidamshina, D. R. Targeting microbial biofilms using ficin, a nonspecific plant protease / D. R.Baidamshina, E.Y.Trizna, M.G.Holyavka, M.I.Bogachev, V.G.Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Sci. Rep. - 2017. - V.7. - P.46068.

Аннотация

Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 mu g/ ml and six-fold at 1000 mu g/ ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells

Ключевые слова

MUCOID PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; BACTERIAL BIOFILMS; PROTEOLYTIC-ENZYMES; BACILLUS-SUBTILIS; ALGINATE LYASE; INHIBITION; INFECTIONS; STRATEGY; EPIDERMIDIS

Название журнала

SCIENTIFIC REPORTS

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https://repository.kpfu.ru/?p_id=159910

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ахатова Фарида Сериковна	ru_RU
dc.contributor.author	Байдамшина Диана Рафисовна	ru_RU
dc.contributor.author	Богачев Михаил Игоревич	ru_RU
dc.contributor.author	Рожина Эльвира Вячеславовна	ru_RU
dc.contributor.author	Тризна Елена Юрьевна	ru_RU
dc.contributor.author	Фахруллин Равиль Фаридович	ru_RU
dc.contributor.author	Тризна Елена Юрьевна	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Baidamshina, D. R. Targeting microbial biofilms using ficin, a nonspecific plant protease / D. R.Baidamshina, E.Y.Trizna, M.G.Holyavka, M.I.Bogachev, V.G.Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Sci. Rep. - 2017. - V.7. - P.46068.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=159910	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 mu g/ ml and six-fold at 1000 mu g/ ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Targeting microbial biofilms using ficin, a nonspecific plant protease	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU