КФУ. Карточка публикации. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images

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FAST AND SIMPLE TOOL FOR THE QUANTIFICATION OF BIOFILM-EMBEDDED CELLS SUB-POPULATIONS FROM FLUORESCENT MICROSCOPIC IMAGES

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2017

Язык

английский

Авторы, сотрудники КФУ

Байдамшина Диана Рафисовна, автор

Богачев Михаил Игоревич, автор

Зеленихин Павел Валерьевич, автор

Каюмов Айрат Рашитович, автор

Муртазина Регина Рауфовна, автор

Тризна Елена Юрьевна, автор

Другие авторы

Markelov O. , автор

Библиографическое описание на языке оригинала

Bogachev M. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images / M.Bogachev, O. Markelov, E. Trizna, D. Baydamshina, P. Zelenikhin, R. Murtazina, A. Kayumov // BioRxiv. - 2017. – P.181495.

Аннотация

Fluorescent staining is a common tool for both quantitative and qualitative assessment of pro- and eukaryotic cells sub-population fractions by using microscopy and flow cytometry. However, direct cell counting by flow cytometry is often limited, for example when working with cells rigidly adhered either to each other or to external surfaces like in bacterial biofilms or adherent cell lines and tissue samples. An alternative approach is provided by using fluorescent microscopy and confocal laser scanning microscopy (CLSM), which enables the evaluation of fractions of cells subpopulations in a given sample. To facilitate the quantitative assessment of cell fractions in microphotographs, we suggest a simple two-step algorithm that combines the cell selection based and the statistical approaches. Based on a series of experimental measurements performed on bacterial and eukaryotic cells under various measurement conditions, we show explicitly that the suggested approach effectively account

Ключевые слова

Biofilm

Название журнала

BioRxiv

URL

https://www.biorxiv.org/content/early/2017/08/29/181495

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https://repository.kpfu.ru/?p_id=174577

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Байдамшина Диана Рафисовна	ru_RU
dc.contributor.author	Богачев Михаил Игоревич	ru_RU
dc.contributor.author	Зеленихин Павел Валерьевич	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Муртазина Регина Рауфовна	ru_RU
dc.contributor.author	Тризна Елена Юрьевна	ru_RU
dc.contributor.author	Markelov O.	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Bogachev M. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images / M.Bogachev, O. Markelov, E. Trizna, D. Baydamshina, P. Zelenikhin, R. Murtazina, A. Kayumov // BioRxiv. - 2017. – P.181495.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=174577	ru_RU
dc.description.abstract	BioRxiv	ru_RU
dc.description.abstract	Fluorescent staining is a common tool for both quantitative and qualitative assessment of pro- and eukaryotic cells sub-population fractions by using microscopy and flow cytometry. However, direct cell counting by flow cytometry is often limited, for example when working with cells rigidly adhered either to each other or to external surfaces like in bacterial biofilms or adherent cell lines and tissue samples. An alternative approach is provided by using fluorescent microscopy and confocal laser scanning microscopy (CLSM), which enables the evaluation of fractions of cells subpopulations in a given sample. To facilitate the quantitative assessment of cell fractions in microphotographs, we suggest a simple two-step algorithm that combines the cell selection based and the statistical approaches. Based on a series of experimental measurements performed on bacterial and eukaryotic cells under various measurement conditions, we show explicitly that the suggested approach effectively account	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU