| Форма представления | Тезисы и материалы конференций в зарубежных журналах и сборниках |
| Год публикации | 2018 |
| Язык | английский |
|
Гумерова Аниса Азатовна, автор
Киясов Андрей Павлович, автор
Ризванов Альберт Анатольевич, автор
Титова Ангелина Андреевна, автор
Титова Марина Александровна, автор
Шафигуллина Айгуль Касыймовна, автор
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Гарипов Ильнур Илгизович, автор
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| Библиографическое описание на языке оригинала |
Garipov I.I. Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells / I.I. Garipov, A.K. Shafigullina, A.A. Titova, M.A. Titova, A.A. Rizvanov, A.A. Gumerova, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, November 2018, Lausanne, Switzerland. - HUMAN GENE THERAPY 27: P. A134-135. |
| Аннотация |
HUMAN GENE THERAPY 27 (2018) |
| Ключевые слова |
hepatic stellate cells, cryopreservation, phenotype, long-term cultivation |
| Название журнала |
HUMAN GENE THERAPY 27 (2018)
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| Ссылка для РПД |
http://dspace.kpfu.ru/xmlui/bitstream/handle/net/146668/ESGCT_1_Garipov_I.pdf?sequence=1&isAllowed=y
|
| URL |
https://www.esgct.eu/Congress/Current-Congress.aspx |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=188567 |
| Файлы ресурса | |
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Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Гумерова Аниса Азатовна |
ru_RU |
| dc.contributor.author |
Киясов Андрей Павлович |
ru_RU |
| dc.contributor.author |
Ризванов Альберт Анатольевич |
ru_RU |
| dc.contributor.author |
Титова Ангелина Андреевна |
ru_RU |
| dc.contributor.author |
Титова Марина Александровна |
ru_RU |
| dc.contributor.author |
Шафигуллина Айгуль Касыймовна |
ru_RU |
| dc.contributor.author |
Гарипов Ильнур Илгизович |
ru_RU |
| dc.date.accessioned |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2018 |
ru_RU |
| dc.identifier.citation |
Garipov I.I. Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells / I.I. Garipov, A.K. Shafigullina, A.A. Titova, M.A. Titova, A.A. Rizvanov, A.A. Gumerova, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, November 2018, Lausanne, Switzerland. - HUMAN GENE THERAPY 27: P. A134-135. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=188567 |
ru_RU |
| dc.description.abstract |
HUMAN GENE THERAPY 27 (2018) |
ru_RU |
| dc.description.abstract |
Many organs and tissues have been reported to harbor regional stem cells. One of the candidates for this role in the liver is hepatic stellate cells (HSC).
Cultivation of cells in vitro may lead to changes in their morphology and phenotype. The risk of changes increases with long-term cultivation (passage 5 and above), as well as with cryopreservation of cells. Freezing allows storage of isolated cell; however, the method can influence cells phenotype and properties.
The aim of research was to study phenotype of rat HSC during long-term cultivation and cryopreservation.
Three cultures of HSC (I ? after cryopreservation, II ? long-term culture, passage 6, and III ? control, passage 4) were stained immunocytochemically with antibodies to desmin, α-SMA, CK19, Ki-67; quantitative real-time PCR analysis was also performed to evaluate desmin and α-SMA gene expression.
It was observed that morphology of cells was similar in all three groups. In groups with long-term cultivation and cryopreser |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
hepatic stellate cells |
ru_RU |
| dc.subject |
cryopreservation |
ru_RU |
| dc.subject |
phenotype |
ru_RU |
| dc.subject |
long-term cultivation |
ru_RU |
| dc.title |
Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells |
ru_RU |
| dc.type |
Тезисы и материалы конференций в зарубежных журналах и сборниках |
ru_RU |
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