| Форма представления | Тезисы и материалы конференций в зарубежных журналах и сборниках |
| Год публикации | 2019 |
| Язык | английский |
|
Калигин Максим Сергеевич, автор
Киясов Андрей Павлович, автор
Мавликеев Михаил Олегович, автор
Ризванов Альберт Анатольевич, автор
Титова Ангелина Андреевна, автор
Чернова Ольга Николаевна, автор
Шафигуллина Айгуль Касыймовна, автор
Яковлева Ольга Владиславовна, автор
|
| Библиографическое описание на языке оригинала |
Shafigullina A.K. Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice / A.K. Shafigullina, M.S. Kaligin, M.O. Mavlikeev, O.N. Chernova, O.V. Yakovleva, A.A. Titova, A.A. Rizvanov, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, 22-25 October 2019, Barcelona, Spain. - HUMAN GENE THERAPY: (P470) – P.156-157. |
| Аннотация |
HUMAN GENE THERAPY |
| Ключевые слова |
streptozocin-nicotinamide, diabetes mellitus, liver, hepatic stellate cells, myofibroblasts, macrophages |
| Название журнала |
HUMAN GENE THERAPY
|
| Ссылка для РПД |
http://dspace.kpfu.ru/xmlui/bitstream/handle/net/151976/F_ESGCT_Aygul_2019_250_words.pdf?sequence=1&isAllowed=y
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| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=213549 |
| Файлы ресурса | |
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Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Калигин Максим Сергеевич |
ru_RU |
| dc.contributor.author |
Киясов Андрей Павлович |
ru_RU |
| dc.contributor.author |
Мавликеев Михаил Олегович |
ru_RU |
| dc.contributor.author |
Ризванов Альберт Анатольевич |
ru_RU |
| dc.contributor.author |
Титова Ангелина Андреевна |
ru_RU |
| dc.contributor.author |
Чернова Ольга Николаевна |
ru_RU |
| dc.contributor.author |
Шафигуллина Айгуль Касыймовна |
ru_RU |
| dc.contributor.author |
Яковлева Ольга Владиславовна |
ru_RU |
| dc.date.accessioned |
2019-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2019-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2019 |
ru_RU |
| dc.identifier.citation |
Shafigullina A.K. Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice / A.K. Shafigullina, M.S. Kaligin, M.O. Mavlikeev, O.N. Chernova, O.V. Yakovleva, A.A. Titova, A.A. Rizvanov, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, 22-25 October 2019, Barcelona, Spain. - HUMAN GENE THERAPY: (P470) – P.156-157. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=213549 |
ru_RU |
| dc.description.abstract |
HUMAN GENE THERAPY |
ru_RU |
| dc.description.abstract |
One of diabetes mellitus (DM) animal models is performed by injection of streptozocin-nicotinamide (SZC-NA) – agents that via GLUT-2 transporter blockage affect glucose transport into cells. GLUT-2 is highly present on pancreatic β-cells that explain toxicity of STZ to them and application of SZN-NA to model DM. GLUT-2 is present also on hepatocytes, which participate in maintaining glucose levels and deposit it as glycogen. Damage of hepatocytes is always accompanied by activation of hepatic stellate cells (HSC) that transform into collagen-producing myofibroblasts, resulting in liver fibrosis. The aim of the research was to study reaction of liver cells to SZN-NA-induced DM in mice.
Experimental groups: 1) control mice (C57Bl/J); 2) mice with intraperitoneal injection of SZC-NA (100mg/kg). Liver paraffin sections (40 days after) were stained by Mallory's trichrome (connective tissue), PAS (glycogen content), immunohistochemically (IHC) with antibodies to CD163 (macrophages), desmin (HSC), α-SMA (myofibroblasts).
Diabetes development was proved by increase of blood sugar and decrease of insulin-secreting β-cells in pancreas. In the liver there was hydropic degeneration of hepatocytes with reduction of glycogen content, mixed polymorphonuclear-mononuclear infiltration including CD163+macrophages. IHC to desmin demonstrated reduced number of desmin+HSC in compare to control group. IHC to α-SMA showed no myofibroblasts. Features of fibrosis were excluded by Mallory's trichrome staining to connective tissue. Thus, SCZ-NA-induced DM is accompanied by damage of glycogen content in hepatocytes, inflammation with Kupffer macrophages activation. The HSC are inhibited, they don`t transform into myofibroblasts, there is no liver fibrosis development.
Work supported by Program of Competitive Growth of KFU. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
streptozocin-nicotinamide |
ru_RU |
| dc.subject |
diabetes mellitus |
ru_RU |
| dc.subject |
liver |
ru_RU |
| dc.subject |
hepatic stellate cells |
ru_RU |
| dc.subject |
myofibroblasts |
ru_RU |
| dc.subject |
macrophages |
ru_RU |
| dc.title |
Reaction of liver cells to streptozocin-nicotinamide-
induced diabetes mellitus in mice |
ru_RU |
| dc.type |
Тезисы и материалы конференций в зарубежных журналах и сборниках |
ru_RU |
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