КФУ. Карточка публикации. Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors

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ACETYLCHOLINESTERASE SENSOR BASED ON POLYELECTROLYTE COMPLEXES WITH DNA INCLUSION FOR THE DETERMINATION OF REVERSIBLE INHIBITORS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2020

Язык

английский

Авторы, сотрудники КФУ

Евтюгин Геннадий Артурович, автор

Иванов Алексей Николаевич, автор

Авторы, аспиранты КФУ

Давлетшина Регина Рудисовна, автор

Библиографическое описание на языке оригинала

R. Davletshina, A. Ivanov, G. Evtugyn, Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors // Electroanalysis. - 2020. - V.32, No 2. - P. 308-316.

Аннотация

The acetylcholinesterase (AChE) biosensor has been developed for the determination of reversible inhibitors applied in the Alzheimer's disease therapy, i. e., Huperzine A (HupA) and galantamine (Gal). For this purpose, glassy carbon electrode (GCE) was first modified with carbon black (CB) and Co phthalocyanine and then polyelectrolyte complex was self‐assembled on its surface by drop casting of reactants and washing. To extend the stability and improve biosensor performance, it was proposed for the first time to use DNA as polyanion in the complex assembly. The DNA showed higher charge density than conventional polyelectrolytes and stabilized the surface coating by adsorption of higher enzyme amount and prevention of its leaching during the biosensor operation. Complex formation and the influence of structural factors were monitored with surface plasmon resonance. Kinetic study showed mixed inhibition of the enzyme within micro‐ and nanomolar range of inhibitor concentrations.

Ключевые слова

acetylcholinesterase biosensor, reversible inhibition, polyelectrolyte complex, Alzheimer's disease drug

Название журнала

ELECTROANALYSIS

URL

https://doi.org/10.1002/elan.201900507

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https://repository.kpfu.ru/?p_id=218766

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Евтюгин Геннадий Артурович	ru_RU
dc.contributor.author	Иванов Алексей Николаевич	ru_RU
dc.contributor.author	Давлетшина Регина Рудисовна	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	R. Davletshina, A. Ivanov, G. Evtugyn, Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors // Electroanalysis. - 2020. - V.32, No 2. - P. 308-316.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=218766	ru_RU
dc.description.abstract	ELECTROANALYSIS	ru_RU
dc.description.abstract	The acetylcholinesterase (AChE) biosensor has been developed for the determination of reversible inhibitors applied in the Alzheimer's disease therapy, i. e., Huperzine A (HupA) and galantamine (Gal). For this purpose, glassy carbon electrode (GCE) was first modified with carbon black (CB) and Co phthalocyanine and then polyelectrolyte complex was self‐assembled on its surface by drop casting of reactants and washing. To extend the stability and improve biosensor performance, it was proposed for the first time to use DNA as polyanion in the complex assembly. The DNA showed higher charge density than conventional polyelectrolytes and stabilized the surface coating by adsorption of higher enzyme amount and prevention of its leaching during the biosensor operation. Complex formation and the influence of structural factors were monitored with surface plasmon resonance. Kinetic study showed mixed inhibition of the enzyme within micro‐ and nanomolar range of inhibitor concentrations.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	acetylcholinesterase biosensor	ru_RU
dc.subject	reversible inhibition	ru_RU
dc.subject	polyelectrolyte complex	ru_RU
dc.subject	Alzheimer's disease drug	ru_RU
dc.title	Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU