КФУ. Карточка публикации. Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application

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MESO-SUBSTITUTED-BODIPY BASED FLUORESCENT BIOMARKER: SPECTRAL CHARACTERISTICS, PHOTOSTABILITY AND POSSIBILITIES FOR PRACTICAL APPLICATION

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2020

Язык

английский

Авторы, сотрудники КФУ

Исламов Даут Ринатович, автор

Каюмов Айрат Рашитович, автор

Павельев Роман Сергеевич, автор

Усачев Константин Сергеевич, автор

Библиографическое описание на языке оригинала

Guseva G.B, Antina E.V, Berezin M.B, Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application//Journal of Photochemistry and Photobiology A: Chemistry. - 2020. - Vol.401, Is.. - Art. № 112783.

Аннотация

A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3',5,5'-tetramethyl-2,2'-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.

Ключевые слова

BODIPY Fluorescent dyes Biomarker Spectral properties Photostability

Название журнала

Journal of Photochemistry and Photobiology A: Chemistry

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088512805&doi=10.1016%2fj.jphotochem.2020.112783&partnerID=40&md5=552788931cef42745735ce329b4c80e0

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https://repository.kpfu.ru/?p_id=237163

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Исламов Даут Ринатович	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Павельев Роман Сергеевич	ru_RU
dc.contributor.author	Усачев Константин Сергеевич	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Guseva G.B, Antina E.V, Berezin M.B, Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application//Journal of Photochemistry and Photobiology A: Chemistry. - 2020. - Vol.401, Is.. - Art. № 112783.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=237163	ru_RU
dc.description.abstract	Journal of Photochemistry and Photobiology A: Chemistry	ru_RU
dc.description.abstract	A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3',5,5'-tetramethyl-2,2'-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU