КФУ. Карточка публикации. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis.

КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

RUS

- EN
- 中文
- ES

INACTIVATION OF THE GENERAL TRANS&SHY;CRIPTION FACTOR TNRA IN BACILLUS SUBTILIS BY PROTEOLYSIS.

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2008

Авторы, сотрудники КФУ

Ильинская Ольга Николаевна, автор

Каюмов Айрат Рашитович, автор

Шарипова Маргарита Рашидовна, автор

Другие авторы

Karl Forchhamer; Annette Heinrich

Библиографическое описание на языке оригинала

A. Kayumov, A. Heinrich, M. Sharipova, O. Iljinskaya, K. Forchhammer. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis // Microbiology. - 2008. - V.154. - P.2348-2355. DOI 10.1099/mic.0.2008/019802-0.

Аннотация

Under conditions of nitrogen limitation, the general transcription factor TnrA in Bacillus subtilis activates the expression of genes involved in assimilation of various nitrogen sources. Previously, TnrA activity has been shown to be controlled by protein-protein interaction with glutamine synthetase, the key enzyme of ammonia assimilation. Furthermore, depending on ATP and 2-oxoglutarate levels, TnrA can bind to the GlnK-AmtB complex. Here, we report that upon transfer of nitrate-grown ceils to combined nitrogen-depleted medium, TnrA is rapidly eliminated from the cells by proteolysis. As long as TnrA is membrane-bound through GlnK-AmtB interaction it seems to be protected from degradation. Upon removal of nitrogen sources, the localization of TnrA becomes cytosolic and degradation occurs. The proteolytic activity against TnrA was detected in the cytosolic fraction but not in the membrane, and its presence does not depend on the nitrogen regime of cell growth. The proteolytic degradation of TnrA as a response to complete nitrogen starvation might represent a novel mechanism of TnrA control in B. subtilis.

Ключевые слова

Bacillus subtilis, transcription factor TnrA.

Название журнала

MICROBIOL-SGM

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=24078

Файлы ресурса

Название файла	Размер (Мб)	Формат
microbiology_2008.pdf	1,10	pdf	посмотреть / скачать

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ильинская Ольга Николаевна	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Шарипова Маргарита Рашидовна	ru_RU
dc.date.accessioned	2008-01-01T00:00:00Z	ru_RU
dc.date.available	2008-01-01T00:00:00Z	ru_RU
dc.date.issued	2008	ru_RU
dc.identifier.citation	A. Kayumov, A. Heinrich, M. Sharipova, O. Iljinskaya, K. Forchhammer. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis // Microbiology. - 2008. - V.154. - P.2348-2355. DOI 10.1099/mic.0.2008/019802-0.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=24078	ru_RU
dc.description.abstract	MICROBIOL-SGM	ru_RU
dc.description.abstract	Under conditions of nitrogen limitation, the general transcription factor TnrA in Bacillus subtilis activates the expression of genes involved in assimilation of various nitrogen sources. Previously, TnrA activity has been shown to be controlled by protein-protein interaction with glutamine synthetase, the key enzyme of ammonia assimilation. Furthermore, depending on ATP and 2-oxoglutarate levels, TnrA can bind to the GlnK-AmtB complex. Here, we report that upon transfer of nitrate-grown ceils to combined nitrogen-depleted medium, TnrA is rapidly eliminated from the cells by proteolysis. As long as TnrA is membrane-bound through GlnK-AmtB interaction it seems to be protected from degradation. Upon removal of nitrogen sources, the localization of TnrA becomes cytosolic and degradation occurs. The proteolytic activity against TnrA was detected in the cytosolic fraction but not in the membrane, and its presence does not depend on the nitrogen regime of cell growth. The proteolytic degradation of TnrA as a response to complete nitrogen starvation might represent a novel mechanism of TnrA control in B. subtilis.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	transcription factor TnrA.	ru_RU
dc.title	Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis.	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU