КФУ. Карточка публикации. Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode

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ELECTROCHEMICAL DNA SENSOR BASED ON ACRIDINE YELLOW ADSORBED ON GLASSY CARBON ELECTRODE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2021

Язык

английский

Авторы, сотрудники КФУ

Евтюгин Геннадий Артурович, автор

Краснова Татьяна Николаевна, автор

Порфирьева Анна Вениаминовна, автор

Рогов Алексей Михайлович, автор

Библиографическое описание на языке оригинала

Kulikova T., Porfireva A., Rogov A., Evtugyn G. (2021) Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode. Sensors, 21(22):7763. https://doi.org/10.3390/s21227763

Аннотация

Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies

Ключевые слова

Carbon black, Cyclic voltammetry, Doxorubicin determination, Drug determination, Electrochemical DNA sensor

Название журнала

Sensors

URL

https://doi.org/10.3390/s21227763

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https://repository.kpfu.ru/?p_id=272319

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Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Евтюгин Геннадий Артурович	ru_RU
dc.contributor.author	Краснова Татьяна Николаевна	ru_RU
dc.contributor.author	Порфирьева Анна Вениаминовна	ru_RU
dc.contributor.author	Рогов Алексей Михайлович	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Kulikova T., Porfireva A., Rogov A., Evtugyn G. (2021) Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode. Sensors, 21(22):7763. https://doi.org/10.3390/s21227763	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=272319	ru_RU
dc.description.abstract	Sensors	ru_RU
dc.description.abstract	Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Carbon black	ru_RU
dc.subject	Cyclic voltammetry	ru_RU
dc.subject	Doxorubicin determination	ru_RU
dc.subject	Drug determination	ru_RU
dc.subject	Electrochemical DNA sensor	ru_RU
dc.title	Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU