КФУ. Карточка публикации. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies

КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

RUS

- EN
- 中文
- ES

OPTIMIZATION OF β-1,4&MDASH;GLUCOSIDASE AND ACID PHOSPHATASE ENZYME METHODS FOR AGRICULTURAL SOIL STUDIES

Форма представления

Тезисы и материалы конференций в зарубежных журналах и сборниках

Год публикации

2018

Язык

английский

Авторы, сотрудники КФУ

Галиева Гульназ Шайхинуровна, автор

Галицкая Полина Юрьевна, автор

Селивановская Светлана Юрьевна, автор

Библиографическое описание на языке оригинала

Galieva G., Selivanovskaya S., Galitskaya P. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies / Galieva G., Selivanovskaya S., Galitskaya P. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM. International Multidisciplinary Scientific Geoconference, - 2018 . - Vol. 18, № 6.4. - P. 235–242.

Аннотация

International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM

Ключевые слова

β-1,4-glucosidase, Acid phosphatase, Buffer pН, Incubation time, Saturation curve, Soil microbial enzyme activities, Soil suspension, Synthetic fluorescent dye-conjugated substrates

Название журнала

International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=274426

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Галиева Гульназ Шайхинуровна	ru_RU
dc.contributor.author	Галицкая Полина Юрьевна	ru_RU
dc.contributor.author	Селивановская Светлана Юрьевна	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Galieva G., Selivanovskaya S., Galitskaya P. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies / Galieva G., Selivanovskaya S., Galitskaya P. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM. International Multidisciplinary Scientific Geoconference, - 2018 . - Vol. 18, № 6.4. - P. 235–242.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=274426	ru_RU
dc.description.abstract	International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM	ru_RU
dc.description.abstract	In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (β-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629 % of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-β-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, the optimal time of measurement was chosen. For β-1,4-glucosidase, it was revealed that 4-MUB-β-d-glucopyranoside concentration should be 400 ?mol g-1 of soil, buffer pH should be 8.0, and soil suspension should be prepared using 1 part of soil and 100 parts of water. The measurements should be done after 60 min of incubation. For acid phosphatase, it was revealed that 4-MUB-phosphate concentration should be 400 ?mol g-1 of soil, buffer pH should be 11.0, and soil suspension should be prepared using 1 part of soil and 50 parts of water. The measurements should be done after 30 min of incubation. ? 2018, International Multidisciplinary Scientific Geoconference. All rights reserved.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	β-1	ru_RU
dc.subject	4-glucosidase	ru_RU
dc.subject	Acid phosphatase	ru_RU
dc.subject	Buffer pН	ru_RU
dc.subject	Incubation time	ru_RU
dc.subject	Saturation curve	ru_RU
dc.subject	Soil microbial enzyme activities	ru_RU
dc.subject	Soil suspension	ru_RU
dc.subject	Synthetic fluorescent dye-conjugated substrates	ru_RU
dc.title	Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies	ru_RU
dc.type	Тезисы и материалы конференций в зарубежных журналах и сборниках	ru_RU