| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2019 |
| Язык | английский |
|
Аганов Альберт Вартанович, автор
Киясов Андрей Павлович, автор
Кочнева Анастасия Анатольевна, автор
Мавликеев Михаил Олегович, автор
Мельникова Анастасия Александровна, автор
|
| Библиографическое описание на языке оригинала |
Lipachev, N. Quantitative changes in perineuronal nets in development and posttraumatic condition [Text] / N. Lipachev, N. Arnst, A. Melnikova, H. Jäälinoja, A. Kochneva, A. Zhigalov, N. Kulesskaya, A.V. Aganov, M. Mavlikeev, H. Rauvala, A.P. Kiyasov, M. Paveliev // Journal of Molecular Histology. – 2019. - Vol. 50, Issue 3. – P. 203–216. |
| Аннотация |
Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity
and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda
agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and
spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14,
21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically
for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin
sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then
addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection
injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase
of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the
single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic
tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing
and posttraumatic CNS. |
| Ключевые слова |
Perineuronal nets ? Extracellular matrix ? Somatosensory cortex ? Brain development ? Spinal cord injury |
| Название журнала |
Journal of Molecular Histology
|
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=279110 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Аганов Альберт Вартанович |
ru_RU |
| dc.contributor.author |
Киясов Андрей Павлович |
ru_RU |
| dc.contributor.author |
Кочнева Анастасия Анатольевна |
ru_RU |
| dc.contributor.author |
Мавликеев Михаил Олегович |
ru_RU |
| dc.contributor.author |
Мельникова Анастасия Александровна |
ru_RU |
| dc.date.accessioned |
2019-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2019-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2019 |
ru_RU |
| dc.identifier.citation |
Lipachev, N. Quantitative changes in perineuronal nets in development and posttraumatic condition [Text] / N. Lipachev, N. Arnst, A. Melnikova, H. Jäälinoja, A. Kochneva, A. Zhigalov, N. Kulesskaya, A.V. Aganov, M. Mavlikeev, H. Rauvala, A.P. Kiyasov, M. Paveliev // Journal of Molecular Histology. – 2019. - Vol. 50, Issue 3. – P. 203–216. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=279110 |
ru_RU |
| dc.description.abstract |
Journal of Molecular Histology |
ru_RU |
| dc.description.abstract |
Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity
and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda
agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and
spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14,
21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically
for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin
sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then
addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection
injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase
of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the
single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic
tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing
and posttraumatic CNS. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
|
ru_RU |
| dc.title |
Quantitative changes in perineuronal nets in development and posttraumatic condition |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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