| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2023 |
| Язык | английский |
|
Закирова Елена Юрьевна, автор
Ризванов Альберт Анатольевич, автор
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Ravilov Rustem , автор
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| Библиографическое описание на языке оригинала |
Ravilov R. Efficient delivery of the immunodominant genes of African swine fever virus by adeno-associated virus serotype 2 / Ravilov R, Galeeva A, Frolov G, Efimova M, Zakirova E, Rizvanov A, Hisamutdinov A, Garipov L, and Mingaleev D // Veterinary World. - 2023. - Т.16. - №12. - р.2425-2430. |
| Аннотация |
Veterinary World |
| Ключевые слова |
adeno-associated virus, African swine fever virus, gene delivery, virally vectored vaccine |
| Название журнала |
Veterinary World
|
| URL |
https://www.researchgate.net/publication/376290676_Efficient_delivery_of_the_immunodominant_genes_of_African_swine_fever_virus_by_adeno-associated_virus_serotype_2 |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=293165 |
| Файлы ресурса | |
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Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Закирова Елена Юрьевна |
ru_RU |
| dc.contributor.author |
Ризванов Альберт Анатольевич |
ru_RU |
| dc.contributor.author |
Ravilov Rustem |
ru_RU |
| dc.date.accessioned |
2023-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2023-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2023 |
ru_RU |
| dc.identifier.citation |
Ravilov R. Efficient delivery of the immunodominant genes of African swine fever virus by adeno-associated virus serotype 2 / Ravilov R, Galeeva A, Frolov G, Efimova M, Zakirova E, Rizvanov A, Hisamutdinov A, Garipov L, and Mingaleev D // Veterinary World. - 2023. - Т.16. - №12. - р.2425-2430. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=293165 |
ru_RU |
| dc.description.abstract |
Veterinary World |
ru_RU |
| dc.description.abstract |
Adeno-associated virus serotype 2 (AAV2) represents a promising basis for developing a virus-vector vaccine against African swine fever (ASF). This study aimed to create genetic constructs based on AAV2 to deliver the immunodominant genes of ASF virus (ASFV) and to evaluate their functionality in vitro. The efficiency and specificity of transgene expression, as well as their non-toxicity in cells of target animals, were evaluated. Materials and Methods: Bioinformatics analysis methods were used to identify the immunodominant genes of ASFV. The target genes B646L, E183L, CP204L, and CP530R were identified and subsequently cloned into the pAAV-MCS vector. Assembly of recombinant AAV2 (rAAV2) was performed by cotransfection of AAV293 cells with the following plasmids: pAAV-MCS with the gene of interest, envelope, and packaging. Quantitative polymerase chain reaction was used to determine the AAV2 titer. The functionality of the constructs was evaluated in HEK293 and SPEV cells by determining the presence of mature proteins in the cell lysate and the expression levels of messenger RNA. The specificity of the target proteins in cell lysates was confirmed by Western blotting. Results: The proposed AAV2 assembly protocol makes it possible to achieve a concentration of mature viral particles of at least 280 billion/mL of virus-containing material. The rAAV2 could effectively transduce host SPEV cells. The expression of both cistrons was detectable during the transduction of cells; therefore, the combined expression of immunogens in the cells of target animals should be possible using this method. Conclusion: This study demonstrated the potential of using genetic constructs based on AAV2 for the delivery of ASFV genes in vitro. Keywords: adeno-associated virus, African swine fever virus, gene delivery, virally vectored vaccine. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
adeno-associated virus |
ru_RU |
| dc.subject |
African swine fever virus |
ru_RU |
| dc.subject |
gene delivery |
ru_RU |
| dc.subject |
virally vectored vaccine |
ru_RU |
| dc.title |
Efficient delivery of the immunodomi-nant genes of African swine fever virus by adeno-associated virus serotype 2 |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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