| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2022 |
| Язык | русский |
|
Даминова Амина Галеевна, автор
Минибаева Фарида Вилевна, автор
|
| Библиографическое описание на языке оригинала |
Daminova A.G., Rogov A.M., Rassabina A.E., Beckett R.P., Minibayeva F.V., Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria / A. G. Daminova ,A. M. Rogov, A. E. Rassabina , R. P. Beckett, F. V. Minibayeva // Journal of Fungi (Basel).- 2022.- V. 8. - № 791.- P. 1-10. |
| Аннотация |
Lichens often grow in microhabitats where they experience severe abiotic stresses. Some species respond to high UV radiation by synthesizing dark brown melanic pigments in the upper cortex. However, unlike the melanized structures of non-lichenized fungi, the morphology of the melanic layer in lichens remains unstudied. Here, we analyzed the morphology, ultrastructure, and elemental composition of the melanized layer in UV-exposed thalli of the lichen Lobaria pulmonaria (L.) Hoffm. Using light microscopy, we detected a pigmented layer sensitive to staining with 3,4-Ldihydroxyphenylalanine, a precursor of eumelanin, in the upper cortex of melanized thalli. Analysis
of cross-sections of melanized thalli using scanning electron microscopy revealed that melanin-like granules are deposited into the hyphal lumens. Melanized thalli also possessed thicker hyphal
cell walls compared to pale thalli. Energy-dispersive X-ray spectroscopy analysis of the elemental composition of the hyphal walls and extracted melanin indicated that the type of melanin synthesized by L. pulmonaria is eumelanin. Transmission electron microscopy was used to show that during melanosome-like dark vesicles are transported to the cell surface and secreted into the cell walls of the fungal hyphae. Results from this study provide new insights into the effects of melanin synthesis on the microstructure of lichen thalli.
|
| Ключевые слова |
lichens, melanins, mycobiont, scanning electron microscopy, transmission electron microscopy, UV stress |
| Название журнала |
Journal of Fungi
|
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=310647 |
| Файлы ресурса | |
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|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Даминова Амина Галеевна |
ru_RU |
| dc.contributor.author |
Минибаева Фарида Вилевна |
ru_RU |
| dc.date.accessioned |
2022-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2022-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2022 |
ru_RU |
| dc.identifier.citation |
Daminova A.G., Rogov A.M., Rassabina A.E., Beckett R.P., Minibayeva F.V., Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria / A. G. Daminova ,A. M. Rogov, A. E. Rassabina , R. P. Beckett, F. V. Minibayeva // Journal of Fungi (Basel).- 2022.- V. 8. - № 791.- P. 1-10. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=310647 |
ru_RU |
| dc.description.abstract |
Journal of Fungi |
ru_RU |
| dc.description.abstract |
Lichens often grow in microhabitats where they experience severe abiotic stresses. Some species respond to high UV radiation by synthesizing dark brown melanic pigments in the upper cortex. However, unlike the melanized structures of non-lichenized fungi, the morphology of the melanic layer in lichens remains unstudied. Here, we analyzed the morphology, ultrastructure, and elemental composition of the melanized layer in UV-exposed thalli of the lichen Lobaria pulmonaria (L.) Hoffm. Using light microscopy, we detected a pigmented layer sensitive to staining with 3,4-Ldihydroxyphenylalanine, a precursor of eumelanin, in the upper cortex of melanized thalli. Analysis
of cross-sections of melanized thalli using scanning electron microscopy revealed that melanin-like granules are deposited into the hyphal lumens. Melanized thalli also possessed thicker hyphal
cell walls compared to pale thalli. Energy-dispersive X-ray spectroscopy analysis of the elemental composition of the hyphal walls and extracted melanin indicated that the type of melanin synthesized by L. pulmonaria is eumelanin. Transmission electron microscopy was used to show that during melanosome-like dark vesicles are transported to the cell surface and secreted into the cell walls of the fungal hyphae. Results from this study provide new insights into the effects of melanin synthesis on the microstructure of lichen thalli.
|
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
lichens |
ru_RU |
| dc.subject |
melanins |
ru_RU |
| dc.subject |
mycobiont |
ru_RU |
| dc.subject |
scanning electron microscopy |
ru_RU |
| dc.subject |
transmission electron microscopy |
ru_RU |
| dc.subject |
UV stress |
ru_RU |
| dc.title |
Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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