КФУ. Карточка публикации. New insight into secreted ribonuclease structure: binase is a natural dimer

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NEW INSIGHT INTO SECRETED RIBONUCLEASE STRUCTURE: BINASE IS A NATURAL DIMER

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2014

Язык

английский

Авторы, сотрудники КФУ

Дудкина Елена Владимировна, автор

Ильинская Ольга Николаевна, автор

Каюмов Айрат Рашитович, автор

Библиографическое описание на языке оригинала

Dudkina E. New insight into secreted ribonuclease structure: binase is a natural dimer / Dudkina E., Kayumov A., Ulyanova V., Ilinskaya O. // PLoS One. - 2014. - V. 9(12). - P. e115818. doi:10.1371/journal.pone.0115818.

Аннотация

The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity.

Ключевые слова

ribonuclease, binase, dimer

Название журнала

PLos ONE

URL

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115818

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https://repository.kpfu.ru/?p_id=88343

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Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Дудкина Елена Владимировна	ru_RU
dc.contributor.author	Ильинская Ольга Николаевна	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Dudkina E. New insight into secreted ribonuclease structure: binase is a natural dimer / Dudkina E., Kayumov A., Ulyanova V., Ilinskaya O. // PLoS One. - 2014. - V. 9(12). - P. e115818. doi:10.1371/journal.pone.0115818.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=88343	ru_RU
dc.description.abstract	PLos ONE	ru_RU
dc.description.abstract	The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	ribonuclease	ru_RU
dc.subject	binase	ru_RU
dc.subject	dimer	ru_RU
dc.title	New insight into secreted ribonuclease structure: binase is a natural dimer	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU