КФУ. Карточка публикации. C-Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization.

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C-TERMINUS OF TRANS&SHY;CRIPTION FACTOR TNRA FROM BACILLUS SUBTILIS CONTROLS DNA-BINDING DOMAIN ACTIVITY BUT IS NOT REQUIRED FOR DIMERIZATION.

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2013

Авторы, сотрудники КФУ

Ильинская Ольга Николаевна, автор

Каюмов Айрат Рашитович, автор

Федорова Ксения Павловна, автор

Другие авторы

M.I. Bogachev; E.Y. Turbina

Библиографическое описание на языке оригинала

K.P. Fedorova, I.S. Scharafutdinov, E.Y. Turbina, M.I. Bogachev, O.N. Ilinskaja, A.R. Kayumov Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization // Molecular Biology. - 2013. - V. 47. - № 2, Р. 293–298.

Аннотация

The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C- terminus controls the DNA-binding activity in a full-length TnrA.

Ключевые слова

Bacillus subtilis, transcription factor TnrA, dimerization.

Название журнала

MOL BIOL REP

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Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ильинская Ольга Николаевна	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Федорова Ксения Павловна	ru_RU
dc.date.accessioned	2013-01-01T00:00:00Z	ru_RU
dc.date.available	2013-01-01T00:00:00Z	ru_RU
dc.date.issued	2013	ru_RU
dc.identifier.citation	K.P. Fedorova, I.S. Scharafutdinov, E.Y. Turbina, M.I. Bogachev, O.N. Ilinskaja, A.R. Kayumov Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization // Molecular Biology. - 2013. - V. 47. - № 2, Р. 293–298.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=90440	ru_RU
dc.description.abstract	MOL BIOL REP	ru_RU
dc.description.abstract	The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C- terminus controls the DNA-binding activity in a full-length TnrA.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	transcription factor TnrA	ru_RU
dc.subject	dimerization.	ru_RU
dc.title	C-Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization.	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU