КФУ. Карточка публикации. Conjugates of a photoactivated rhodamine with biopolymers for cell staining

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CONJUGATES OF A PHOTOACTIVATED RHODAMINE WITH BIOPOLYMERS FOR CELL STAINING

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2014

Язык

английский

Авторы, сотрудники КФУ

Соловьева Валерия Владимировна, автор

Библиографическое описание на языке оригинала

Zaitsev S.Y. Conjugates of a photoactivated rhodamine with biopolymers for cell staining / S.Y. Zaitsev, M.N. Shaposhnikov, D.O. Solovyeva, V.V. Solovyeva, A.A. Rizvanov // The Scientific World Journal. – 2014. – V.2014. - Article ID 285405. – doi: 10.1155/2014/285405.

Аннотация

Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (?Chitosan-PFD?) and histone H1 (?Histone H1.3-PFD?). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (?caged? dyes) for microscopic probing of biological objects. Thus, the synthesized ?Chitosan-PFD? and ?Histone H1-PFD? have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.

Ключевые слова

photoactivated rhodamine dyes, histone H1.3

Название журнала

The scientific world journal

URL

http://www.hindawi.com/journals/tswj/2014/285405/

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https://repository.kpfu.ru/?p_id=93684

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Соловьева Валерия Владимировна	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Zaitsev S.Y. Conjugates of a photoactivated rhodamine with biopolymers for cell staining / S.Y. Zaitsev, M.N. Shaposhnikov, D.O. Solovyeva, V.V. Solovyeva, A.A. Rizvanov // The Scientific World Journal. – 2014. – V.2014. - Article ID 285405. – doi: 10.1155/2014/285405.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=93684	ru_RU
dc.description.abstract	The scientific world journal	ru_RU
dc.description.abstract	Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (?Chitosan-PFD?) and histone H1 (?Histone H1.3-PFD?). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (?caged? dyes) for microscopic probing of biological objects. Thus, the synthesized ?Chitosan-PFD? and ?Histone H1-PFD? have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	photoactivated rhodamine dyes	ru_RU
dc.subject	histone H1.3	ru_RU
dc.title	Conjugates of a photoactivated rhodamine with biopolymers for cell staining	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU