KFU. Card publication. Lentivirus Expression of Hantavirus Nucleocapsid Proteins

KAZAN
FEDERAL UNIVERSITY

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LENTIVIRUS EXPRESSION OF HANTAVIRUS NUCLEOCAPSID PROTEINS

Form of presentation

Articles in international journals and collections

Year of publication

2016

Язык

английский

Authors, employees of KFU

Garanina Ekaterina Evgenevna, author

Muyangva Musalva , author

Rizvanov Albert Anatolevich, author

Bibliographic description in the original language

Muyangwa M, GaraninaE. E., MartynovaE. V. , RizvanovA. A. Lentivirus Expression of Hantavirus Nucleocapsid Proteins.BioNanoScience. 2016 August 27.doi:10.1007/s12668-016-0250-9

Annotation

Hantaviruses are classified as category A pathogens due to the high mortality rate and potential for major health impact. Therefore, virus propagation is restricted to the Biological Safety Level-3 containment laboratory. In this work, we utilized the recombinant lentivirus delivery system which can be used in Biological Safety Level-2 containment. Recombinant lentiviruses were obtained using Gateway cloning technology. Lentiviruses containing S segment of Hantaan, Sin Nombre, Prospect Hill, or Andes viruses were used to transduce lung carcinoma epithelial type II cells (A549). Accumulation of hantavirus S segment RNA was detected using real-time PCR. Additionally, expression of hantavirus nucleocapsid protein was detected in cells transduced with lentiviruses coding for hantavirus S segment. Hantavirus N proteins were found localized in perinuclear region. Interestingly, localization of N protein was similar to that found in hantavirus infected cells. Therefore, we suggest that lentiv

Keywords

Recombinant Lentivirus,Nucleocapsid protein,Hantavirus, Immunohistochemistry

The name of the journal

BioNanoScience

URL

http:///ink.springer.com/article/10.1007/s12668-016-0250-9

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=142999&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Garanina Ekaterina Evgenevna	ru_RU
dc.contributor.author	Muyangva Musalva	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Muyangwa M, GaraninaE. E., MartynovaE. V. , RizvanovA. A. Lentivirus Expression of Hantavirus Nucleocapsid Proteins.BioNanoScience. 2016 August 27.doi:10.1007/s12668-016-0250-9	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=142999&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Hantaviruses are classified as category A pathogens due to the high mortality rate and potential for major health impact. Therefore, virus propagation is restricted to the Biological Safety Level-3 containment laboratory. In this work, we utilized the recombinant lentivirus delivery system which can be used in Biological Safety Level-2 containment. Recombinant lentiviruses were obtained using Gateway cloning technology. Lentiviruses containing S segment of Hantaan, Sin Nombre, Prospect Hill, or Andes viruses were used to transduce lung carcinoma epithelial type II cells (A549). Accumulation of hantavirus S segment RNA was detected using real-time PCR. Additionally, expression of hantavirus nucleocapsid protein was detected in cells transduced with lentiviruses coding for hantavirus S segment. Hantavirus N proteins were found localized in perinuclear region. Interestingly, localization of N protein was similar to that found in hantavirus infected cells. Therefore, we suggest that lentiv	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Recombinant Lentivirus	ru_RU
dc.subject	Nucleocapsid protein	ru_RU
dc.subject	Hantavirus	ru_RU
dc.subject	Immunohistochemistry	ru_RU
dc.title	Lentivirus Expression of Hantavirus Nucleocapsid Proteins	ru_RU
dc.type	Articles in international journals and collections	ru_RU