КФУ. Карточка публикации. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene

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AN IMPROVED GENE EXPRESSION SYSTEM TO GENERATE TRANSGENIC ARABIDOPSIS THALIANA PLANTS HARBORING A BACILLUS GINSENGIHUMI PHYTASE GENE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2015

Авторы, сотрудники КФУ

Валеева Лия Рашитовна, автор

Нямсурэн Чулуунцэцэг ., автор

Шакиров Евгений Витальевич, автор

Шарипова Маргарита Рашидовна, автор

Авторы, аспиранты КФУ

Валеева Лия Рашитовна, автор

Библиографическое описание на языке оригинала

Nyamsuren, Ch. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene / Ch. Nyamsuren, L. R Valeeva, M. R Sharipova, E. V Shakirov // RJPBCS. ? 2015. ? V.6(4). - P.18-24.

Аннотация

We constructed a new vector system for heterologous gene expression in Arabidopsis thaliana. The construct contains a codon-optimized sequence encoding Bacillus ginsengihumi phytase behind an inducible plant-specific promoter for expression in root epithelial cells. The new vector introduced into the plant A. thaliana by Agrobacterium mediated transformation. We obtained several generations of transgenic A. thaliana plants with integrated Bacillus ginsengihumi phytase gene, as well as with an empty vector as a negative control. We tested several transgenic plants harboring the phyCg construct under the control of phosphate-starvation inducible Pht1;2 promoter and show that the phyCg gene is expressed at the mRNA level. Further characterization of these lines of plants will help us to design an improved transgenic strategy for the development of a root-specific heterologous system for the expression of bacterial phytases in plants.

Ключевые слова

Bacillus phytase, Pht1;2 promoter, Arabidopsis thaliana

Название журнала

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6(4)/[4].pdf

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https://repository.kpfu.ru/?p_id=125196

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Валеева Лия Рашитовна	ru_RU
dc.contributor.author	Нямсурэн Чулуунцэцэг .	ru_RU
dc.contributor.author	Шакиров Евгений Витальевич	ru_RU
dc.contributor.author	Шарипова Маргарита Рашидовна	ru_RU
dc.contributor.author	Валеева Лия Рашитовна	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Nyamsuren, Ch. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene / Ch. Nyamsuren, L. R Valeeva, M. R Sharipova, E. V Shakirov // RJPBCS. ? 2015. ? V.6(4). - P.18-24.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=125196	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	We constructed a new vector system for heterologous gene expression in Arabidopsis thaliana. The construct contains a codon-optimized sequence encoding Bacillus ginsengihumi phytase behind an inducible plant-specific promoter for expression in root epithelial cells. The new vector introduced into the plant A. thaliana by Agrobacterium mediated transformation. We obtained several generations of transgenic A. thaliana plants with integrated Bacillus ginsengihumi phytase gene, as well as with an empty vector as a negative control. We tested several transgenic plants harboring the phyCg construct under the control of phosphate-starvation inducible Pht1;2 promoter and show that the phyCg gene is expressed at the mRNA level. Further characterization of these lines of plants will help us to design an improved transgenic strategy for the development of a root-specific heterologous system for the expression of bacterial phytases in plants.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus phytase	ru_RU
dc.subject	Pht1;2 promoter	ru_RU
dc.subject	Arabidopsis thaliana	ru_RU
dc.title	An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU