КФУ. Карточка публикации. Heterologous expression of Bacillus ginsengihumi phytase gene

КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

RUS

- EN
- 中文
- ES

HETEROLOGOUS EXPRESSION OF BACILLUS GINSENGIHUMI PHYTASE GENE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2015

Язык

английский

Авторы, сотрудники КФУ

Ахметова Алина Ильдусовна, автор

Балабан Нэлли Павловна, автор

Сулейманова Алия Дамировна, автор

Тойменцева Анна Александровна, автор

Шарипова Маргарита Рашидовна, автор

Авторы, студенты КФУ

Илюхина Дарья Леонидовна, автор

Библиографическое описание на языке оригинала

A. Akhmetova. Heterologous expression of Bacillus ginsengihumi phytase gene / A. Akhmetova, A. Suleimanova, A. Toymentseva, N. Balaban, D. Iljukhina, M. Sharipova // Research Journal of Pharmaceutical, Biological and Chemical Science.- 2015.- 6(4).-P. 117-122

Аннотация

Phytate is the most abundant reservoir of phosphorus, which can potentially be used by microorganisms, plants and animals. Microbial phytases, capable of hydrolysing insoluble phytates, can be used as animal feed additives. In the present study, we isolated new phytase-producing bacteria - Bacillus ginsengihumi M2.11, which was identified using Microbial Identification System and 16S rRNA sequencing. We first cloned and sequenced the 1149 bp open reading frame encoding Bacillus ginsengihumi M2.11 phytase into the expression vector pET-46 Ek/LIC by the LIC-cloning method (cloning without ligase). The recombinant E.coli strain expressing Bacillus ginsengihumi M2.11 phytase was obtained. The molecular weight of phytase was 41 kDa

Ключевые слова

phytase, cloning, Bacillus, expression

Название журнала

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6%284%29/%5B19%5D.pdf

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=125891

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ахметова Алина Ильдусовна	ru_RU
dc.contributor.author	Балабан Нэлли Павловна	ru_RU
dc.contributor.author	Сулейманова Алия Дамировна	ru_RU
dc.contributor.author	Тойменцева Анна Александровна	ru_RU
dc.contributor.author	Шарипова Маргарита Рашидовна	ru_RU
dc.contributor.author	Илюхина Дарья Леонидовна	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	A. Akhmetova. Heterologous expression of Bacillus ginsengihumi phytase gene / A. Akhmetova, A. Suleimanova, A. Toymentseva, N. Balaban, D. Iljukhina, M. Sharipova // Research Journal of Pharmaceutical, Biological and Chemical Science.- 2015.- 6(4).-P. 117-122	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=125891	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	Phytate is the most abundant reservoir of phosphorus, which can potentially be used by microorganisms, plants and animals. Microbial phytases, capable of hydrolysing insoluble phytates, can be used as animal feed additives. In the present study, we isolated new phytase-producing bacteria - Bacillus ginsengihumi M2.11, which was identified using Microbial Identification System and 16S rRNA sequencing. We first cloned and sequenced the 1149 bp open reading frame encoding Bacillus ginsengihumi M2.11 phytase into the expression vector pET-46 Ek/LIC by the LIC-cloning method (cloning without ligase). The recombinant E.coli strain expressing Bacillus ginsengihumi M2.11 phytase was obtained. The molecular weight of phytase was 41 kDa	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	phytase	ru_RU
dc.subject	cloning	ru_RU
dc.subject	Bacillus	ru_RU
dc.subject	expression	ru_RU
dc.title	Heterologous expression of Bacillus ginsengihumi phytase gene	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU