КФУ. Карточка публикации. Lentivirus Expression of Hantavirus Nucleocapsid Proteins

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LENTIVIRUS EXPRESSION OF HANTAVIRUS NUCLEOCAPSID PROTEINS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2016

Язык

английский

Авторы, сотрудники КФУ

Гаранина Екатерина Евгеньевна, автор

Муйангва Мусалва , автор

Ризванов Альберт Анатольевич, автор

Библиографическое описание на языке оригинала

Muyangwa M, GaraninaE. E., MartynovaE. V. , RizvanovA. A. Lentivirus Expression of Hantavirus Nucleocapsid Proteins.BioNanoScience. 2016 August 27.doi:10.1007/s12668-016-0250-9

Аннотация

Hantaviruses are classified as category A pathogens due to the high mortality rate and potential for major health impact. Therefore, virus propagation is restricted to the Biological Safety Level-3 containment laboratory. In this work, we utilized the recombinant lentivirus delivery system which can be used in Biological Safety Level-2 containment. Recombinant lentiviruses were obtained using Gateway cloning technology. Lentiviruses containing S segment of Hantaan, Sin Nombre, Prospect Hill, or Andes viruses were used to transduce lung carcinoma epithelial type II cells (A549). Accumulation of hantavirus S segment RNA was detected using real-time PCR. Additionally, expression of hantavirus nucleocapsid protein was detected in cells transduced with lentiviruses coding for hantavirus S segment. Hantavirus N proteins were found localized in perinuclear region. Interestingly, localization of N protein was similar to that found in hantavirus infected cells. Therefore, we suggest that lentiv

Ключевые слова

Recombinant Lentivirus,Nucleocapsid protein,Hantavirus, Immunohistochemistry

Название журнала

BioNanoScience

URL

http:///ink.springer.com/article/10.1007/s12668-016-0250-9

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https://repository.kpfu.ru/?p_id=142999

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Гаранина Екатерина Евгеньевна	ru_RU
dc.contributor.author	Муйангва Мусалва	ru_RU
dc.contributor.author	Ризванов Альберт Анатольевич	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Muyangwa M, GaraninaE. E., MartynovaE. V. , RizvanovA. A. Lentivirus Expression of Hantavirus Nucleocapsid Proteins.BioNanoScience. 2016 August 27.doi:10.1007/s12668-016-0250-9	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=142999	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Hantaviruses are classified as category A pathogens due to the high mortality rate and potential for major health impact. Therefore, virus propagation is restricted to the Biological Safety Level-3 containment laboratory. In this work, we utilized the recombinant lentivirus delivery system which can be used in Biological Safety Level-2 containment. Recombinant lentiviruses were obtained using Gateway cloning technology. Lentiviruses containing S segment of Hantaan, Sin Nombre, Prospect Hill, or Andes viruses were used to transduce lung carcinoma epithelial type II cells (A549). Accumulation of hantavirus S segment RNA was detected using real-time PCR. Additionally, expression of hantavirus nucleocapsid protein was detected in cells transduced with lentiviruses coding for hantavirus S segment. Hantavirus N proteins were found localized in perinuclear region. Interestingly, localization of N protein was similar to that found in hantavirus infected cells. Therefore, we suggest that lentiv	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Recombinant Lentivirus	ru_RU
dc.subject	Nucleocapsid protein	ru_RU
dc.subject	Hantavirus	ru_RU
dc.subject	Immunohistochemistry	ru_RU
dc.title	Lentivirus Expression of Hantavirus Nucleocapsid Proteins	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU