| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2017 |
| Язык | английский |
|
Зайнутдинова Эльмира Фаритовна, автор
Романова Юлия Джафаровна, автор
Филимонова Мария Николаевна, автор
|
|
Губская Валентина Петровна, автор
|
| Библиографическое описание на языке оригинала |
Romanova J. Analysis of the mechanism of Mg2+ action on the RNase activity of Serratia marcescens endonuclease //
J.Romanova, V. Gubskaya, I.Nuretdinov, E. Zainutdinova, M. Filimonova//BioNanoScience. -2017.-V.2.- P.276-283
|
| Аннотация |
The main goal of the investigation was to analyzeto analyze the mechanism of Mg2+ action on the digestive activity of Serratia marcescens nuclease towards RNA due to the potential application of the nuclease as a reagent for removing nucleic acids from biomedical samples as well as an antiviral factor. Examination of the mechanism revealed that it was similar to the mechanism of the metals actions on the DNase activity. The optimal Mg2+ amount was linked with the changing secondary structure of RNA - substrates within A-helix. Addition of Mg2+ was found to affect both the rates of products dissociations from the enzyme-substrate complexes and the enzyme associations with the substrates, that was supported by strong increase in the Kcat values and change in the Km values . values. Comparing the mechanisms of Mg2+ action on RNase and DNase activities in S. marcescens nuclease, we identified a preference for DNA in the absence of Mg2+ shown that was supported by with the higher Kcat values |
| Ключевые слова |
Serratia marcescens nuclease, the mechanism of Mg2+ action on the RNase activity ,activity, preference for DNA substrate |
| Название журнала |
BioNanoScience
|
| URL |
http://link.springer.com/article/10.1007/s12668-016-0358-y |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=146403 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Зайнутдинова Эльмира Фаритовна |
ru_RU |
| dc.contributor.author |
Романова Юлия Джафаровна |
ru_RU |
| dc.contributor.author |
Филимонова Мария Николаевна |
ru_RU |
| dc.contributor.author |
Губская Валентина Петровна |
ru_RU |
| dc.date.accessioned |
2017-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2017-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2017 |
ru_RU |
| dc.identifier.citation |
Romanova J. Analysis of the mechanism of Mg2+ action on the RNase activity of Serratia marcescens endonuclease //
J.Romanova, V. Gubskaya, I.Nuretdinov, E. Zainutdinova, M. Filimonova//BioNanoScience. -2017.-V.2.- P.276-283
|
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=146403 |
ru_RU |
| dc.description.abstract |
BioNanoScience |
ru_RU |
| dc.description.abstract |
The main goal of the investigation was to analyzeto analyze the mechanism of Mg2+ action on the digestive activity of Serratia marcescens nuclease towards RNA due to the potential application of the nuclease as a reagent for removing nucleic acids from biomedical samples as well as an antiviral factor. Examination of the mechanism revealed that it was similar to the mechanism of the metals actions on the DNase activity. The optimal Mg2+ amount was linked with the changing secondary structure of RNA - substrates within A-helix. Addition of Mg2+ was found to affect both the rates of products dissociations from the enzyme-substrate complexes and the enzyme associations with the substrates, that was supported by strong increase in the Kcat values and change in the Km values . values. Comparing the mechanisms of Mg2+ action on RNase and DNase activities in S. marcescens nuclease, we identified a preference for DNA in the absence of Mg2+ shown that was supported by with the higher Kcat values |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Serratia marcescens nuclease |
ru_RU |
| dc.subject |
the mechanism of Mg2+ action on the RNase activity |
ru_RU |
| dc.subject |
activity |
ru_RU |
| dc.subject |
preference for DNA substrate |
ru_RU |
| dc.title |
Analysis of the mechanism of Mg2+ action on the RNase activity of Serratia marcescens endonuclease |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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