| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Хайрутдинов Булат Имамутдинович, автор
|
|
Ermakova Elena , автор
Nesmelova Irina , автор
|
| Библиографическое описание на языке оригинала |
Ermakova E.A. Structure of Scots pine defensine 1 by spectroscopic methods and computational modeling / E.A. Ermakova, D.A. Faizullin, B.Z. Idiyatullin, B.I. Khairutdinov, L.N. Mukhamedova, N.B. Tarasova, Y.Y. Toporkova, E.V. Osipova, Y.V. Gogolev, Y.F. Zuev, I.V. Nesmelova // International Journal of Biologocal Macromolecules. – 2016. Vol. 84. – P. 142-152. |
| Аннотация |
Defensins are part of the innate immune system in plants with activity against a broad range of pathogens,including bacteria, fungi and viruses. Several defensins from conifers, including Scots pine defensin 1(Pinus sylvestris defensin 1, (PsDef1)) have shown a strong antifungal activity, however structural andphysico-chemical properties of the family, needed for establishing the structure-dynamics-function rela-tionships, remain poorly characterized. We use several spectroscopic and computational methods tocharacterize the structure, dynamics, and oligomeric state of PsDef1. The three-dimensional structure wasmodeled by comparative modeling using several programs (Geno3D, SWISS-MODEL, I-TASSER, Phyre2,and FUGUE) and verified by circular dichroism (CD) and infrared (FTIR) spectroscopy. Furthermore, FTIRdata indicates that the structure of PsDef1 is highly resistant to high temperatures. NMR diffusion experi-ments show that defensin exists in solution in the equilibrium between monomers |
| Ключевые слова |
Defensin, Structure, Dimer, Computational modeling, Circular dichroism, FTIRa |
| Название журнала |
INT J BIOL MACROMOL
|
| URL |
http://www.sciencedirect.com/science/article/pii/S0141813015301987 |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=147298 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Хайрутдинов Булат Имамутдинович |
ru_RU |
| dc.contributor.author |
Ermakova Elena |
ru_RU |
| dc.contributor.author |
Nesmelova Irina |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Ermakova E.A. Structure of Scots pine defensine 1 by spectroscopic methods and computational modeling / E.A. Ermakova, D.A. Faizullin, B.Z. Idiyatullin, B.I. Khairutdinov, L.N. Mukhamedova, N.B. Tarasova, Y.Y. Toporkova, E.V. Osipova, Y.V. Gogolev, Y.F. Zuev, I.V. Nesmelova // International Journal of Biologocal Macromolecules. – 2016. Vol. 84. – P. 142-152. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=147298 |
ru_RU |
| dc.description.abstract |
INT J BIOL MACROMOL |
ru_RU |
| dc.description.abstract |
Defensins are part of the innate immune system in plants with activity against a broad range of pathogens,including bacteria, fungi and viruses. Several defensins from conifers, including Scots pine defensin 1(Pinus sylvestris defensin 1, (PsDef1)) have shown a strong antifungal activity, however structural andphysico-chemical properties of the family, needed for establishing the structure-dynamics-function rela-tionships, remain poorly characterized. We use several spectroscopic and computational methods tocharacterize the structure, dynamics, and oligomeric state of PsDef1. The three-dimensional structure wasmodeled by comparative modeling using several programs (Geno3D, SWISS-MODEL, I-TASSER, Phyre2,and FUGUE) and verified by circular dichroism (CD) and infrared (FTIR) spectroscopy. Furthermore, FTIRdata indicates that the structure of PsDef1 is highly resistant to high temperatures. NMR diffusion experi-ments show that defensin exists in solution in the equilibrium between monomers |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Defensin |
ru_RU |
| dc.subject |
Structure |
ru_RU |
| dc.subject |
Dimer |
ru_RU |
| dc.subject |
Computational modeling |
ru_RU |
| dc.subject |
Circular dichroism |
ru_RU |
| dc.subject |
FTIRa |
ru_RU |
| dc.title |
Structure of Scots pine defensine 1 by spectroscopic methods and computational modeling |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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