| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Шафигуллина Айгуль Касыймовна, автор
|
|
Albrecht Ute , автор
Groos-Sahr Katerina , автор
Görg Boris , автор
Häussinger Dieter , автор
Karababa Ayse , автор
Keitel Verena , автор
|
| Библиографическое описание на языке оригинала |
Karababa, A. Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia / A. Karababa, K. Groos-Sahr, U. Albrecht, V. Keitel, A. Shafigullina, B. Görg, D. Häussinger // Neurochem Res. - 2016. - Р. 1–13. |
| Аннотация |
Hepatic encephalopathy (HE) is associated with cerebral microglia activation. Ammonia, a major toxin of HE, activates microglia in vitro but does not trigger pro-inflammatory cytokine synthesis. In the present study we analysed effects of ammonia on lipopolysaccharide (LPS)-induced upregulation of microglia activation and cytokine mRNA as well as on cytokine secretion in mono-cultured microglia and co-cultured astrocytes and microglia. In mono-cultured microglia LPS (100 ng/ml, 18 h) strongly elevated mRNA levels of the microglia activation marker CD14 and the pro-inflammatory cytokines IL-1α/β, IL-6 and TNF-α. NH4Cl (5 mmol/l) had no effect on LPS-induced upregulation of CD14, IL-1α/β and IL-6 mRNA but enhanced LPS-induced upregulation of TNF-α mRNA in mono-cultured microglia. In co-cultured astrocytes and microglia, however, LPS-induced upregulation of IL-1α/β, TNF-α, IL-6, CD14 but not of IL-10, IL-12A/B or TGFβ1-3 mRNA was attenuated by NH4Cl. LPS-induced upregulation of IL-1α/β, I |
| Ключевые слова |
Ammonia; Hepatic encephalopathy; Neuroinflammation; Neurosteroids; TGR5 |
| Название журнала |
Neurochem Res.
|
| Ссылка для РПД |
http://dspace.kpfu.ru/xmlui/bitstream/handle/net/108584/Ammonia_Attenuates_LPS_Induced_Karababa_2016.pdf?sequence=1&isAllowed=y
|
| URL |
https://www.ncbi.nlm.nih.gov/pubmed/?term=Ammonia+Attenuates+LPS-Induced+Upregulation+of+Pro-Inflammatory+Cytokine+mRNA+in+Co-Cultured+Astrocytes+and+Microglia |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=148271 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Шафигуллина Айгуль Касыймовна |
ru_RU |
| dc.contributor.author |
Albrecht Ute |
ru_RU |
| dc.contributor.author |
Groos-Sahr Katerina |
ru_RU |
| dc.contributor.author |
Görg Boris |
ru_RU |
| dc.contributor.author |
Häussinger Dieter |
ru_RU |
| dc.contributor.author |
Karababa Ayse |
ru_RU |
| dc.contributor.author |
Keitel Verena |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Karababa, A. Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia / A. Karababa, K. Groos-Sahr, U. Albrecht, V. Keitel, A. Shafigullina, B. Görg, D. Häussinger // Neurochem Res. - 2016. - Р. 1–13. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=148271 |
ru_RU |
| dc.description.abstract |
Neurochem Res. |
ru_RU |
| dc.description.abstract |
Hepatic encephalopathy (HE) is associated with cerebral microglia activation. Ammonia, a major toxin of HE, activates microglia in vitro but does not trigger pro-inflammatory cytokine synthesis. In the present study we analysed effects of ammonia on lipopolysaccharide (LPS)-induced upregulation of microglia activation and cytokine mRNA as well as on cytokine secretion in mono-cultured microglia and co-cultured astrocytes and microglia. In mono-cultured microglia LPS (100 ng/ml, 18 h) strongly elevated mRNA levels of the microglia activation marker CD14 and the pro-inflammatory cytokines IL-1α/β, IL-6 and TNF-α. NH4Cl (5 mmol/l) had no effect on LPS-induced upregulation of CD14, IL-1α/β and IL-6 mRNA but enhanced LPS-induced upregulation of TNF-α mRNA in mono-cultured microglia. In co-cultured astrocytes and microglia, however, LPS-induced upregulation of IL-1α/β, TNF-α, IL-6, CD14 but not of IL-10, IL-12A/B or TGFβ1-3 mRNA was attenuated by NH4Cl. LPS-induced upregulation of IL-1α/β, I |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
|
ru_RU |
| dc.title |
Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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