КФУ. Карточка публикации. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene

RUS

- EN
- 中文
- ES

SPECIES-SPECIFIC DIFFERENCES IN PEROXISOME PROLIFERATION, CATALASE, AND SOD2 UPREGULATION AS WELL AS TOXICITY IN HUMAN, MOUSE, AND RAT HEPATOMA CELLS INDUCED BY THE EXPLOSIVE AND ENVIRONMENTAL POLLUTANT 2,4,6-TRINITROTOLUENE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2017

Язык

русский

Авторы, сотрудники КФУ

Науменко Екатерина Анатольевна, автор

Библиографическое описание на языке оригинала

Naumenko, E.A. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene / E.A. Naumenko , B. Ahlemeyer, E. Baumgart-Vogt // Environ Toxicol. – 2017. – V. 32(3) – P. 989-1006

Аннотация

2,4,6-Trinitrotoluene (TNT) has been widely used as an explosive substance and its toxicity is still of interest as it persisted in polluted areas. TNT is metabolized in hepatocytes which are prone to its toxicity. Since analysis of the human liver or hepatocytes is restricted due to ethical reasons, we investigated the effects of TNT on cell viability, reactive oxygen species (ROS) production, peroxisome proliferation, and antioxidative enzymes in human (HepG2), mouse (Hepa 1-6), and rat (H4IIEC3) hepatoma cell lines. Under control conditions, hepatoma cells of all three species were highly comparable exhibiting identical proliferation rates and distribution of their cell cycle phases. However, we found strong differences in TNT toxicity with the lowest IC50 values (highest cell death rate) for rat cells, whereas human and mouse cells were three to sevenfold less sensitive. Moreover, a strong decrease in cellular dehydrogenase activity (MTT assay) and increased ROS levels were noted.

Ключевые слова

Species-specific differences, peroxisome proliferation, 2,4,6-trinitrotoluene

Название журнала

Environmental Toxicology

URL

http://onlinelibrary.wiley.com/doi/10.1002/tox.22299/abstract;jsessionid=0C4AB62C762812F187CE16E65C5BD21E.f03t04

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=159814

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Науменко Екатерина Анатольевна	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Naumenko, E.A. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene / E.A. Naumenko , B. Ahlemeyer, E. Baumgart-Vogt // Environ Toxicol. – 2017. – V. 32(3) – P. 989-1006	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=159814	ru_RU
dc.description.abstract	Environmental Toxicology	ru_RU
dc.description.abstract	2,4,6-Trinitrotoluene (TNT) has been widely used as an explosive substance and its toxicity is still of interest as it persisted in polluted areas. TNT is metabolized in hepatocytes which are prone to its toxicity. Since analysis of the human liver or hepatocytes is restricted due to ethical reasons, we investigated the effects of TNT on cell viability, reactive oxygen species (ROS) production, peroxisome proliferation, and antioxidative enzymes in human (HepG2), mouse (Hepa 1-6), and rat (H4IIEC3) hepatoma cell lines. Under control conditions, hepatoma cells of all three species were highly comparable exhibiting identical proliferation rates and distribution of their cell cycle phases. However, we found strong differences in TNT toxicity with the lowest IC50 values (highest cell death rate) for rat cells, whereas human and mouse cells were three to sevenfold less sensitive. Moreover, a strong decrease in cellular dehydrogenase activity (MTT assay) and increased ROS levels were noted.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Species-specific differences	ru_RU
dc.subject	peroxisome proliferation	ru_RU
dc.subject	2	ru_RU
dc.subject	4	ru_RU
dc.subject	6-trinitrotoluene	ru_RU
dc.title	Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU