| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Абдулкина Лилия Ринатовна, автор
|
| Библиографическое описание на языке оригинала |
Nigmatullina, L.R. Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants / L.R. Nigmatullina, M.R. Sharipova, E.V. Shakirov // BioNanoScience.- 2016.- V. 6.- I. 4.-P. 325–328. |
| Аннотация |
The length of telomeric DNA is often considered a cellular biomarker of aging and general health
status. Several telomere length measuring assays have been developed, of which the most common is the Telomere
Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide
probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive
waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number
of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana
and present evidence that this approach can be used successfully to efficiently and accurately measure telomere
length in plants. Specifically, hybridization temperature of 42 C instead of the typical 55 C appears to generate
stronger signals. In addition, DIG incorporation at 5'-end instead of 3'-end of the labeled oligonucleotide great |
| Ключевые слова |
Nonradioactive telomere restriction fragment analysis, Southern blot, DNA, Aging, Plant |
| Название журнала |
BioNanoScience
|
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=160114 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Абдулкина Лилия Ринатовна |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Nigmatullina, L.R. Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants / L.R. Nigmatullina, M.R. Sharipova, E.V. Shakirov // BioNanoScience.- 2016.- V. 6.- I. 4.-P. 325–328. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=160114 |
ru_RU |
| dc.description.abstract |
BioNanoScience |
ru_RU |
| dc.description.abstract |
The length of telomeric DNA is often considered a cellular biomarker of aging and general health
status. Several telomere length measuring assays have been developed, of which the most common is the Telomere
Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide
probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive
waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number
of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana
and present evidence that this approach can be used successfully to efficiently and accurately measure telomere
length in plants. Specifically, hybridization temperature of 42 C instead of the typical 55 C appears to generate
stronger signals. In addition, DIG incorporation at 5'-end instead of 3'-end of the labeled oligonucleotide great |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Nonradioactive telomere restriction fragment analysis |
ru_RU |
| dc.subject |
Southern blot |
ru_RU |
| dc.subject |
DNA |
ru_RU |
| dc.subject |
Aging |
ru_RU |
| dc.subject |
Plant |
ru_RU |
| dc.title |
Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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