КФУ. Карточка публикации. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine

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REACTIVATION OF SERRATIA MARCESCENS MUTANT ENDONUCLEASE BY HYDROXILAMINE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2018

Язык

английский

Авторы, сотрудники КФУ

Гимадутдинов Олег Александрович, автор

Трушин Максим Викторович, автор

Хамидуллина Раиса Гусмановна, автор

Авторы, студенты КФУ

Фазлеева Ильмира Ильдаровна, автор

Библиографическое описание на языке оригинала

R.G. Khamidullina. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine/R.G. Khamidullina, I.I. Fazleeva, M.V.Trushin, O.A. Gimadutdinov///J. Pharm. Sci. & Res. Vol. 10(9), 2018, 2341-2345

Аннотация

It is known that histidine plays an important role in the catalytic activity of many nucleases. Performing the function of a common base of these enzymes, it activates the formation of hydroxyl from the water molecule, which, in turn, by attacking the phosphorus atom of the diester bond causes its rupture. It was previously shown that in the endonuclease Serratia marcescens, the replacement of histidine with glycine results in its inactivation. We were able to restore the hydroxylamine activity of the mutant enzyme Serratia marcescens endonuclease, in which histidine in the 89th position is replaced by glycine.

Ключевые слова

Key words: endonuclease, plasmid, basal level of expression, hydroxylamine, histidine.

Название журнала

Journal of Pharmaceutical Sciences and Research

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https://repository.kpfu.ru/?p_id=187009

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Гимадутдинов Олег Александрович	ru_RU
dc.contributor.author	Трушин Максим Викторович	ru_RU
dc.contributor.author	Хамидуллина Раиса Гусмановна	ru_RU
dc.contributor.author	Фазлеева Ильмира Ильдаровна	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	R.G. Khamidullina. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine/R.G. Khamidullina, I.I. Fazleeva, M.V.Trushin, O.A. Gimadutdinov///J. Pharm. Sci. & Res. Vol. 10(9), 2018, 2341-2345	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=187009	ru_RU
dc.description.abstract	Journal of Pharmaceutical Sciences and Research	ru_RU
dc.description.abstract	It is known that histidine plays an important role in the catalytic activity of many nucleases. Performing the function of a common base of these enzymes, it activates the formation of hydroxyl from the water molecule, which, in turn, by attacking the phosphorus atom of the diester bond causes its rupture. It was previously shown that in the endonuclease Serratia marcescens, the replacement of histidine with glycine results in its inactivation. We were able to restore the hydroxylamine activity of the mutant enzyme Serratia marcescens endonuclease, in which histidine in the 89th position is replaced by glycine.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Key words: endonuclease	ru_RU
dc.subject	plasmid	ru_RU
dc.subject	basal level of expression	ru_RU
dc.subject	hydroxylamine	ru_RU
dc.subject	histidine.	ru_RU
dc.title	Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU