| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2018 |
| Язык | английский |
|
Шуралев Эдуард Аркадьевич, автор
|
|
Косарев М. А., автор
Осянин К. А., автор
Хаммадов Н. И., автор
|
|
Хаммадова Альфия Василевна, автор
|
| Библиографическое описание на языке оригинала |
Khammadova A.V., Khammadov N.I., Kosarev M.A., Osyanin K.A., Shuralev E.A. Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR // International Journal of Engineering and Technology(UAE). - 2018. - Vol.7, Is.4.7 Special Issue 7. - P.227-230. |
| Аннотация |
Based on the identified genes, the design of specific primers and probes for qPCR was made, which makes it possible to differentiate B.
melitensis and B. abortus in a single reaction. For the differentiation of Brucella strains of the same species, the PCR method of multilocus
VNTR analysis, optimized for uniform temperature conditions, is described. In addition, MLVA profiles for B. abortus and B.
melitensis genomes are presented. All methods of indication and differentiation of Brucella species and strains are described in detail,
which allows them to be used in the everyday work of any PCR laboratory dealing with brucellosis. Control of amplification in the indication
of bacteria of the genus Brucella and determination of their belonging to B. abortus and B. melitensis species is recommended to
be carried out using the plasmid DNA designed in this work. |
| Ключевые слова |
Brucella, genome, indication, nano particle, identification, qPCR, MLVA |
| Название журнала |
International Journal of Engineering and Technology(UAE)
|
| URL |
https://www.scopus.com/record/display.uri?eid=2-s2.0-85075105336&origin=inward&txGid=bc752bc06069daf694435e0af8873d17 |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=187192 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Шуралев Эдуард Аркадьевич |
ru_RU |
| dc.contributor.author |
Косарев М. А. |
ru_RU |
| dc.contributor.author |
Осянин К. А. |
ru_RU |
| dc.contributor.author |
Хаммадов Н. И. |
ru_RU |
| dc.contributor.author |
Хаммадова Альфия Василевна |
ru_RU |
| dc.date.accessioned |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2018 |
ru_RU |
| dc.identifier.citation |
Khammadova A.V., Khammadov N.I., Kosarev M.A., Osyanin K.A., Shuralev E.A. Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR // International Journal of Engineering and Technology(UAE). - 2018. - Vol.7, Is.4.7 Special Issue 7. - P.227-230. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=187192 |
ru_RU |
| dc.description.abstract |
International Journal of Engineering and Technology(UAE) |
ru_RU |
| dc.description.abstract |
Based on the identified genes, the design of specific primers and probes for qPCR was made, which makes it possible to differentiate B.
melitensis and B. abortus in a single reaction. For the differentiation of Brucella strains of the same species, the PCR method of multilocus
VNTR analysis, optimized for uniform temperature conditions, is described. In addition, MLVA profiles for B. abortus and B.
melitensis genomes are presented. All methods of indication and differentiation of Brucella species and strains are described in detail,
which allows them to be used in the everyday work of any PCR laboratory dealing with brucellosis. Control of amplification in the indication
of bacteria of the genus Brucella and determination of their belonging to B. abortus and B. melitensis species is recommended to
be carried out using the plasmid DNA designed in this work. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Brucella |
ru_RU |
| dc.subject |
genome |
ru_RU |
| dc.subject |
indication |
ru_RU |
| dc.subject |
nano particle |
ru_RU |
| dc.subject |
identification |
ru_RU |
| dc.subject |
qPCR |
ru_RU |
| dc.subject |
MLVA |
ru_RU |
| dc.title |
Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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