КФУ. Карточка публикации. Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase

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ELECTROCHEMICAL BIOSENSOR BASED ON POLYELECTROLYTE COMPLEXES FOR THE DETERMINATION OF REVERSIBLE INHIBITORS OF ACETYLCHOLINESTERASE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2019

Авторы, сотрудники КФУ

Иванов Алексей Николаевич, автор

Другие авторы

Шарафиева Индира

Авторы, аспиранты КФУ

Давлетшина Регина Рудисовна, автор

Библиографическое описание на языке оригинала

Alexey Ivanov, Regina Davletshina, Indira Sharafieva and Gennady Evtugyn, Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase, Talanta, https://doi.org/10.1016/j.talanta.2018.10.100

Аннотация

Reversible inhibitors of acetylcholinesterase are increasingly applied in the Alzheimer's disease therapy. Their determination with biosensors requires special attention to the enzyme immobilization due to importance of electrostatic and steric factors affecting accessibility of the active site to the substrate and inhibitor present simultaneously. In this work, polyelectrolyte complexes have been assembled from electrochemically inactive components with implementation of acetylcholinesterase from electric eel. The influence of the complex components and enzyme position on the assembling efficiency was explored using surface plasmon resonance and kinetic parameters of the enzyme with amperometry with Co phtalocyanine mediator. The biosensor developed showed robust response toward donepezil and berberine with limit of detection of 0.46 and 70 nM, respectively, within 1 minute after inhibitor addition. The sensitivity of determination coincides or is better than characteristics of biosen

Ключевые слова

acetylcholinesterase biosensor,reversible inhibition,polyelectrolyte complex,Alzheimer's disease drug

Место издания

Netherlands

Название журнала

TALANTA

URL

https://doi.org/10.1016/j.talanta.2018.10.100

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https://repository.kpfu.ru/?p_id=187678

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Иванов Алексей Николаевич	ru_RU
dc.contributor.author	Давлетшина Регина Рудисовна	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Alexey Ivanov, Regina Davletshina, Indira Sharafieva and Gennady Evtugyn, Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase, Talanta, https://doi.org/10.1016/j.talanta.2018.10.100	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=187678	ru_RU
dc.description.abstract	TALANTA	ru_RU
dc.description.abstract	Reversible inhibitors of acetylcholinesterase are increasingly applied in the Alzheimer's disease therapy. Their determination with biosensors requires special attention to the enzyme immobilization due to importance of electrostatic and steric factors affecting accessibility of the active site to the substrate and inhibitor present simultaneously. In this work, polyelectrolyte complexes have been assembled from electrochemically inactive components with implementation of acetylcholinesterase from electric eel. The influence of the complex components and enzyme position on the assembling efficiency was explored using surface plasmon resonance and kinetic parameters of the enzyme with amperometry with Co phtalocyanine mediator. The biosensor developed showed robust response toward donepezil and berberine with limit of detection of 0.46 and 70 nM, respectively, within 1 minute after inhibitor addition. The sensitivity of determination coincides or is better than characteristics of biosen	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	acetylcholinesterase biosensor	ru_RU
dc.subject	reversible inhibition	ru_RU
dc.subject	polyelectrolyte complex	ru_RU
dc.subject	Alzheimer's disease drug	ru_RU
dc.title	Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU