| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2018 |
| Язык | английский |
|
Минибаева Фарида Вилевна, автор
Часов Андрей Васильевич, автор
|
|
Beckett Peter Beckett, автор
Викторова Лариса Викторовна, автор
Трифонова Татьяна Владимировна, автор
|
|
Онеле Алфред Обинна , автор
|
| Библиографическое описание на языке оригинала |
Onele A.O. Biochemical characterization of peroxidases from the moss Dicranum scoparium / A.O. Onele, A. Chasov, L. Viktorova, R.P. Beckett, T. Trifonova, F. Minibayeva // South African Journal of Botany. - 2018. - V. 119. - P. 132-141. |
| Аннотация |
Mosses are a convenient model to study stress responses of plants because of their remarkable stress tolerance.
Peroxidase (EC 1.11.1.7) activities were tested in three moss species, namely Dicranum scoparium, Hylocomium
splendens and Pleurozium schreberi growing together in the same location in a boreal forest. Peroxidase activity
in D. scoparium was twice as high as in other mosses. Total peroxidase activity in unstressed D. scoparium was
constitutively high; furthermore, long-term desiccation caused a significant increase in activity after 48 h of drying. Interestingly, when thalli desiccated for a week were rapidly rehydrated, peroxidase activity initially declined
and then increased after 2 h rehydration. Diverse anionic and cationic isoforms were detected by native isoelectric focusing and PAGE of both crude extracts and partially purified peroxidases. The ability of peroxidases from D.
scoparium to produce superoxide radical (O2 ?−) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5-
sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride
(NBT) staining; specific O2 ?− producing isoforms were revealed using 2D electrophoresis. Given a quinone and
chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (?OH), and production was increased
by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic ?OH
production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity
that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms
displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce
and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress
tolerance. |
| Ключевые слова |
Dicranum scoparium Hedw., Bryophytes, Mosses, Desiccation tolerance, Peroxidase isoforms, Superoxide radical |
| Название журнала |
South African Journal of Botany
|
| URL |
https://www.sciencedirect.com/science/article/pii/S0254629918310251?via%3Dihub |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=202763 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Минибаева Фарида Вилевна |
ru_RU |
| dc.contributor.author |
Часов Андрей Васильевич |
ru_RU |
| dc.contributor.author |
Beckett Peter Beckett |
ru_RU |
| dc.contributor.author |
Викторова Лариса Викторовна |
ru_RU |
| dc.contributor.author |
Трифонова Татьяна Владимировна |
ru_RU |
| dc.contributor.author |
Онеле Алфред Обинна |
ru_RU |
| dc.date.accessioned |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2018-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2018 |
ru_RU |
| dc.identifier.citation |
Onele A.O. Biochemical characterization of peroxidases from the moss Dicranum scoparium / A.O. Onele, A. Chasov, L. Viktorova, R.P. Beckett, T. Trifonova, F. Minibayeva // South African Journal of Botany. - 2018. - V. 119. - P. 132-141. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=202763 |
ru_RU |
| dc.description.abstract |
South African Journal of Botany |
ru_RU |
| dc.description.abstract |
Mosses are a convenient model to study stress responses of plants because of their remarkable stress tolerance.
Peroxidase (EC 1.11.1.7) activities were tested in three moss species, namely Dicranum scoparium, Hylocomium
splendens and Pleurozium schreberi growing together in the same location in a boreal forest. Peroxidase activity
in D. scoparium was twice as high as in other mosses. Total peroxidase activity in unstressed D. scoparium was
constitutively high; furthermore, long-term desiccation caused a significant increase in activity after 48 h of drying. Interestingly, when thalli desiccated for a week were rapidly rehydrated, peroxidase activity initially declined
and then increased after 2 h rehydration. Diverse anionic and cationic isoforms were detected by native isoelectric focusing and PAGE of both crude extracts and partially purified peroxidases. The ability of peroxidases from D.
scoparium to produce superoxide radical (O2 ?−) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5-
sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride
(NBT) staining; specific O2 ?− producing isoforms were revealed using 2D electrophoresis. Given a quinone and
chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (?OH), and production was increased
by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic ?OH
production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity
that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms
displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce
and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress
tolerance. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Dicranum scoparium Hedw. |
ru_RU |
| dc.subject |
Bryophytes |
ru_RU |
| dc.subject |
Mosses |
ru_RU |
| dc.subject |
Desiccation tolerance |
ru_RU |
| dc.subject |
Peroxidase isoforms |
ru_RU |
| dc.subject |
Superoxide radical |
ru_RU |
| dc.title |
Biochemical characterization of peroxidases from the moss Dicranum scoparium |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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