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CELLULAR UPTAKE AND CYTOTOXICITY OF UNMODIFIED PR3+:LAF 3 NANOPARTICLES
Форма представленияСтатьи в зарубежных журналах и сборниках
Год публикации2019
Языканглийский
  • Евтюгин Владимир Геннадьевич, автор
  • Зеленихин Павел Валерьевич, автор
  • Низамутдинов Алексей Сергеевич, автор
  • Пудовкин Максим Сергеевич, автор
  • Сальников Вадим Владимирович, автор
  • Семашко Вадим Владимирович, автор
  • Shtyreva Victoria , автор
  • Библиографическое описание на языке оригинала Pudovkin M.S. Cellular uptake and cytotoxicity of unmodified Pr3+:LaF 3 nanoparticles / Maksim S. Pudovkin, Pavel V. Zelenikhin, Victoria V. Shtyreva,Vladimir G. Evtugyn, Vadim V. Salnikov, Alexey S. Nizamutdinov, Vadim V. Semashko // J Nanopart Res.(2019) 21:184
    Аннотация Pr3+:LaF 3 (C Pr = 1%) nanoparticles were characterized by means of transmission electron micros-copy (TEM), X-ray diffraction, energy-dispersive spec-troscopy, and optical spectroscopy. The obtained 14 nm Pr3+:LaF 3 (C Pr = 1%) crystalline hexagonal-structured nanoparticles contain Pr, La, and F only. The lumines-cent spectra emission bands corresponded to the emission bands of Pr3+ions. The Pr3+:LaF 3 (C Pr = 1%) nano-particles effectively interact with A 549, LE С , and MDCK cells. By means of TEM, it was revealed that after 2 h of the nanoparticle exposure, A 549, MDCK, and LE С cells internalized the nanoparticles and 20 –300 nm agglomerates of the nanoparticles packed into 200 – 500 nm vesicles were found into the cytoplasm. It seems that the internalization occurs via macropinocytosis. In A 549 cells, some vesicles were disrupted and the nanoparticles escaped the vesicles floating freely in the cytoplasm. Flow cytometry showed that all the cells effectively interact with nanoparticles. This interaction leads to cell granularity change. Specifically, A 549, MDCK, and LE С , and cells treated by nanoparticles have the values of size scattered signal 16 ? 2, 20 ? 3, and 39 ? 3%, respectively, com-paring with the untreated cells. The Pr3+:LaF 3 (C Pr = 1%) nanoparticles were not found into the cellular or-ganelles. The cytotoxicity of the Pr3+:LaF 3 (C Pr = 1%) nanoparticles is not significant at concentrations of 0.05,0.1, 0.25, and 0.5 g/L
    Ключевые слова Pr3+:LaF 3 nanoparticles . Fluoride nanoparticles . Cytotoxicity. Cellular uptake . Flow cytometry. Macropinocytosis . Nanobiomedicine
    Название журнала J NANOPART RES
    URL https://link.springer.com/article/10.1007/s11051-019-4628-9
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