КФУ. Карточка публикации. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus

КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

RUS

- EN
- 中文
- ES

INDUCTION OF APOPTOSIS AND AUTOPHAGY IN T-LYMPHOCYTES OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

Форма представления

Статьи в российских журналах и сборниках

Год публикации

2020

Язык

английский

Авторы, сотрудники КФУ

Абрамов Сергей Николаевич, автор

Абрамова Зинаида Ивановна, автор

Андрианова Изабелла Александровна, автор

Биктагирова Эльнара Маулетовна, автор

Ибрагимов Булат Рафисович, автор

Скибо Юлия Валерьевна, автор

Фатхуллина Алия Ринатовна, автор

Авторы, аспиранты КФУ

Ибрагимов Булат Рафисович, автор

Библиографическое описание на языке оригинала

Skibo Y.V. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus/Y.V. Skibo, A.R. Fathullina, B.R.Ibragimov, S.N.Abramov, R.R. Ismagilova, E.M. Biktagirova, I.A. Andrianova1, A.N. Maksudova, Z.I. Abramova // Kazan Medical Journal.- 2020.-V. 101, No. 3.- P.1-8

Аннотация

Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus.Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann?Whitney test.Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found.Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.

Ключевые слова

Bcl-2, caspase-3, Beclin 1, Vps34, LC3, apoptosis, autophagy, systemic lupus erythematosus

Название журнала

Kazan Medical Journal

URL

https://kazanmedjournal.ru/kazanmedj/article/view/25798/pdf_en

Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку

https://repository.kpfu.ru/?p_id=234180

Файлы ресурса

Название файла	Размер (Мб)	Формат
Skibo_KazMedZhurnal25798_62859_1_PB__1_.pdf	0,57	pdf	посмотреть / скачать

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Абрамов Сергей Николаевич	ru_RU
dc.contributor.author	Абрамова Зинаида Ивановна	ru_RU
dc.contributor.author	Андрианова Изабелла Александровна	ru_RU
dc.contributor.author	Биктагирова Эльнара Маулетовна	ru_RU
dc.contributor.author	Ибрагимов Булат Рафисович	ru_RU
dc.contributor.author	Скибо Юлия Валерьевна	ru_RU
dc.contributor.author	Фатхуллина Алия Ринатовна	ru_RU
dc.contributor.author	Ибрагимов Булат Рафисович	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Skibo Y.V. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus/Y.V. Skibo, A.R. Fathullina, B.R.Ibragimov, S.N.Abramov, R.R. Ismagilova, E.M. Biktagirova, I.A. Andrianova1, A.N. Maksudova, Z.I. Abramova // Kazan Medical Journal.- 2020.-V. 101, No. 3.- P.1-8	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=234180	ru_RU
dc.description.abstract	Kazan Medical Journal	ru_RU
dc.description.abstract	Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus.Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann?Whitney test.Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found.Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bcl-2	ru_RU
dc.subject	caspase-3	ru_RU
dc.subject	Beclin 1	ru_RU
dc.subject	Vps34	ru_RU
dc.subject	LC3	ru_RU
dc.subject	apoptosis	ru_RU
dc.subject	autophagy	ru_RU
dc.subject	systemic lupus erythematosus	ru_RU
dc.title	Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus	ru_RU
dc.type	Статьи в российских журналах и сборниках	ru_RU