КФУ. Карточка публикации. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro

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REAPPRAISAL OF ANOXIC SPREADING DEPOLARIZATION AS A TERMINAL EVENT DURING OXYGEN?GLUCOSE DEPRIVATION IN BRAIN SLICES IN VITRO

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2021

Язык

русский

Авторы, сотрудники КФУ

Гайнутдинов Азат Робертович, автор

Мухтаров Марат Рахимзянович, автор

Хазипов Рустем Нариманович, автор

Авторы, аспиранты КФУ

Юзекаева Эльвира Разилевна, автор

Библиографическое описание на языке оригинала

Juzekaeva E. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro/ E. Juzekaeva, A. Gainutdinov, M. Mukhtarov, R. Khazipov // Scientific Reports.-2020. (doi.org/10.1038/s41598-020-75975-w)

Аннотация

Anoxic spreading depolarization (aSD) has been hypothesized as a terminal event during oxygen? glucose deprivation (OGD) in submerged cortical slices in vitro. However, mechanical artifacts caused by aSD-triggered edema may introduce error in the assessment of neuronal viability. Here, using continuous patch-clamp recordings from submerged rat cortical slices, we first confirmed that vast majority of L4 neurons permanently lost their membrane potential during OGD-induced aSD. In some recordings, spontaneous transition from whole-cell to out-side out configuration occurred during or after aSD, and only a small fraction of neurons survived aSD with reperfusion started shortly after aSD. Secondly, to minimize artifacts caused by OGD-induced edema, cells were short-term patched following OGD episodes of various duration. Nearly half of L4 cells maintained membrane potential and showed the ability to spike-fire if reperfusion started less than 10 min after aSD.

Ключевые слова

Anoxic spreading depolarization (aSD), oxygen?glucose deprivation (OGD), submerged rat cortical slices, ischemia, in vitro ischemia model

Название журнала

SCIENTIFIC REPORTS

URL

https://doi.org/10.1038/s41598-020-75975-w

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https://repository.kpfu.ru/?p_id=250013

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Гайнутдинов Азат Робертович	ru_RU
dc.contributor.author	Мухтаров Марат Рахимзянович	ru_RU
dc.contributor.author	Хазипов Рустем Нариманович	ru_RU
dc.contributor.author	Юзекаева Эльвира Разилевна	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Juzekaeva E. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro/ E. Juzekaeva, A. Gainutdinov, M. Mukhtarov, R. Khazipov // Scientific Reports.-2020. (doi.org/10.1038/s41598-020-75975-w)	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=250013	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	Anoxic spreading depolarization (aSD) has been hypothesized as a terminal event during oxygen? glucose deprivation (OGD) in submerged cortical slices in vitro. However, mechanical artifacts caused by aSD-triggered edema may introduce error in the assessment of neuronal viability. Here, using continuous patch-clamp recordings from submerged rat cortical slices, we first confirmed that vast majority of L4 neurons permanently lost their membrane potential during OGD-induced aSD. In some recordings, spontaneous transition from whole-cell to out-side out configuration occurred during or after aSD, and only a small fraction of neurons survived aSD with reperfusion started shortly after aSD. Secondly, to minimize artifacts caused by OGD-induced edema, cells were short-term patched following OGD episodes of various duration. Nearly half of L4 cells maintained membrane potential and showed the ability to spike-fire if reperfusion started less than 10 min after aSD.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Anoxic spreading depolarization (aSD)	ru_RU
dc.subject	oxygen?glucose deprivation (OGD)	ru_RU
dc.subject	submerged rat cortical slices	ru_RU
dc.subject	ischemia	ru_RU
dc.subject	in vitro ischemia model	ru_RU
dc.title	Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU