КФУ. Карточка публикации. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract

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IDENTIFICATION OF GENES INVOLVED IN THE FORMATION OF SOLUBLE DIETARY FIBER IN WINTER RYE GRAIN AND THEIR EXPRESSION IN CULTIVARS WITH DIFFERENT VISCOSITIES OF WHOLE MEAL WATER EXTRACT

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2022

Язык

английский

Авторы, сотрудники КФУ

Пономарева Мира Леонидовна, автор

Библиографическое описание на языке оригинала

Kozlova L.V. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract / L.V. Kozlova, A.R. Nazipova, O.V. Gorshkov, L.F. Gilmullina, O.V. Sautkina, N.V. Petrova, O.I. Trofimova, S.N. Ponomarev, M.L. Ponomareva, T.A. Gorshova // The Crop Journal - 2022. V.10. I. 2 - Pp. 532-549

Аннотация

The grain of rye (Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides, making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract. However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of mRNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides, whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes

Ключевые слова

Rye (Secale cereale) Kernel development Arabinoxylan Mixed-linkage glucan Viscosity

Название журнала

The Crop Journal

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https://repository.kpfu.ru/?p_id=261645

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Пономарева Мира Леонидовна	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Kozlova L.V. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract / L.V. Kozlova, A.R. Nazipova, O.V. Gorshkov, L.F. Gilmullina, O.V. Sautkina, N.V. Petrova, O.I. Trofimova, S.N. Ponomarev, M.L. Ponomareva, T.A. Gorshova // The Crop Journal - 2022. V.10. I. 2 - Pp. 532-549	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=261645	ru_RU
dc.description.abstract	The Crop Journal	ru_RU
dc.description.abstract	The grain of rye (Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides, making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract. However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of mRNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides, whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU