КФУ. Карточка публикации. Polyelectrolyte Polyethylenimine-DNA Complexes in the Composition of Voltammetric Sensors for Detecting DNA Damage

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POLYELECTROLYTE POLYETHYLENIMINE-DNA COMPLEXES IN THE COMPOSITION OF VOLTAMMETRIC SENSORS FOR DETECTING DNA DAMAGE

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2022

Язык

английский

Авторы, сотрудники КФУ

Евтюгин Геннадий Артурович, автор

Зиятдинова Гузель Камилевна, автор

Иванов Алексей Николаевич, автор

Кузин Юрий Иванович, автор

Маланина Анастасия Николаевна, автор

Стойков Иван Иванович, автор

Шурпик Дмитрий Николаевич, автор

Авторы, аспиранты КФУ

Маланина Анастасия Николаевна, автор

Библиографическое описание на языке оригинала

Malanina, A.N. Polyelectrolyte polyethylenimine-DNA complexes in the composition of voltammetric sensors for detecting DNA damage / A.N. Malanina, Yu. I. Kuzin, A.N. Ivanov, G.K. Ziyatdinova, D.N. Shurpik, I.I. Stoikov, G.A. Evtugyn // J. Anal. Chem. - 2022. - V. 77. - № 2. - P. 185-194.

Аннотация

A voltammetric DNA sensor based on the polyelectrolyte complexes of polyethylenimine and DNA obtained by self-assembly from solutions of components on a glassy carbon electrode has been developed. Cyclic voltammetry was used to study the preparation conditions and characterize the permeability of the complex for low-molecular-weight redox indicators differing in the mechanism of interaction with DNA (methylene blue and methylene green). The formation of the polyelectrolyte complex was independently confirmed by surface plasmon resonance measurements. The redox peak currents of methylene blue and methylene green varied differently depending on the number of layers of the complex and the nature of the outer layer due to different mechanisms of binding the indicators to DNA molecules and varied assembly density of the complex polyelectrolytes. An increase in the concentration of polyions from 0.1 to 10 μg/mL suppressed the effect of the charge of the outer layer on the indicator currents. Polyelectrolyte coatings were tested to detect specific DNA interactions including intercalation with the anticancer drug doxorubicin and the thermal and chemical denaturation of DNA. It was found that the introduction of decacarboxylated pillar[5]arene into the layer increased the sensitivity of the methylene blue signal to the thermal denaturation of DNA. A comparison between changes in the peak currents of methylene blue and methylene green makes it possible to distinguish the mechanism of DNA damage and separately take into account the contribution of the considered chemical and physical damaging factors. The developed DNA sensor makes it possible to semiquantitatively evaluate the protective effect of antioxidants, which decrease the effect of reactive oxygen species on DNA in the biosensor.

Ключевые слова

DNA sensor, polyelectrolyte complexes, voltammetry, oxidative DNA damage, methylene blue, methylene green

Название журнала

JOURNAL OF ANALYTICAL CHEMISTRY

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125589325&doi=10.1134%2fS1061934822020095&partnerID=40&md5=36b5b205a5f6e263396a17ee82f73b56

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https://repository.kpfu.ru/?p_id=264199

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Евтюгин Геннадий Артурович	ru_RU
dc.contributor.author	Зиятдинова Гузель Камилевна	ru_RU
dc.contributor.author	Иванов Алексей Николаевич	ru_RU
dc.contributor.author	Кузин Юрий Иванович	ru_RU
dc.contributor.author	Маланина Анастасия Николаевна	ru_RU
dc.contributor.author	Стойков Иван Иванович	ru_RU
dc.contributor.author	Шурпик Дмитрий Николаевич	ru_RU
dc.contributor.author	Маланина Анастасия Николаевна	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Malanina, A.N. Polyelectrolyte polyethylenimine-DNA complexes in the composition of voltammetric sensors for detecting DNA damage / A.N. Malanina, Yu. I. Kuzin, A.N. Ivanov, G.K. Ziyatdinova, D.N. Shurpik, I.I. Stoikov, G.A. Evtugyn // J. Anal. Chem. - 2022. - V. 77. - № 2. - P. 185-194.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=264199	ru_RU
dc.description.abstract	JOURNAL OF ANALYTICAL CHEMISTRY	ru_RU
dc.description.abstract	A voltammetric DNA sensor based on the polyelectrolyte complexes of polyethylenimine and DNA obtained by self-assembly from solutions of components on a glassy carbon electrode has been developed. Cyclic voltammetry was used to study the preparation conditions and characterize the permeability of the complex for low-molecular-weight redox indicators differing in the mechanism of interaction with DNA (methylene blue and methylene green). The formation of the polyelectrolyte complex was independently confirmed by surface plasmon resonance measurements. The redox peak currents of methylene blue and methylene green varied differently depending on the number of layers of the complex and the nature of the outer layer due to different mechanisms of binding the indicators to DNA molecules and varied assembly density of the complex polyelectrolytes. An increase in the concentration of polyions from 0.1 to 10 μg/mL suppressed the effect of the charge of the outer layer on the indicator currents. Polyelectrolyte coatings were tested to detect specific DNA interactions including intercalation with the anticancer drug doxorubicin and the thermal and chemical denaturation of DNA. It was found that the introduction of decacarboxylated pillar[5]arene into the layer increased the sensitivity of the methylene blue signal to the thermal denaturation of DNA. A comparison between changes in the peak currents of methylene blue and methylene green makes it possible to distinguish the mechanism of DNA damage and separately take into account the contribution of the considered chemical and physical damaging factors. The developed DNA sensor makes it possible to semiquantitatively evaluate the protective effect of antioxidants, which decrease the effect of reactive oxygen species on DNA in the biosensor.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	DNA sensor	ru_RU
dc.subject	polyelectrolyte complexes	ru_RU
dc.subject	voltammetry	ru_RU
dc.subject	oxidative DNA damage	ru_RU
dc.subject	methylene blue	ru_RU
dc.subject	methylene green	ru_RU
dc.title	Polyelectrolyte Polyethylenimine-DNA Complexes in the Composition of Voltammetric Sensors for Detecting DNA Damage	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU