КФУ. Карточка публикации. Isolation, culturing and 3D bioprinting equine myoblasts

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ISOLATION, CULTURING AND 3D BIOPRINTING EQUINE MYOBLASTS

Форма представления

Статьи в российских журналах и сборниках

Год публикации

2022

Язык

английский

Авторы, сотрудники КФУ

Аймалетдинов Александр Маазович, автор

Закирова Елена Юрьевна, автор

Ризванов Альберт Анатольевич, автор

Другие авторы

Rutland Catrin , автор

Авторы, студенты КФУ

Курилов Игорь Витальевич, автор

Юферова Алина Константиновна, автор

Библиографическое описание на языке оригинала

Aimaletdinov A. М. Isolation, culturing and 3D bioprinting equine myoblasts / А.М.Aimaletdinov, М. Abyzova, I. Kurilov, A. Yuferova, C. Rutland, A.A. Rizvanov, E.Yu. Zakirova // Biological Communications. - 2022. - № 67(3). - р.152–159.

Аннотация

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.

Ключевые слова

myoblasts, bioprinting, microcarriers

Название журнала

Biological Communications

URL

https://doi.org/10.21638/spbu03.2022.302

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https://repository.kpfu.ru/?p_id=272340

Файлы ресурса

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Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Аймалетдинов Александр Маазович	ru_RU
dc.contributor.author	Закирова Елена Юрьевна	ru_RU
dc.contributor.author	Ризванов Альберт Анатольевич	ru_RU
dc.contributor.author	Rutland Catrin	ru_RU
dc.contributor.author	Курилов Игорь Витальевич	ru_RU
dc.contributor.author	Юферова Алина Константиновна	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Aimaletdinov A. М. Isolation, culturing and 3D bioprinting equine myoblasts / А.М.Aimaletdinov, М. Abyzova, I. Kurilov, A. Yuferova, C. Rutland, A.A. Rizvanov, E.Yu. Zakirova // Biological Communications. - 2022. - № 67(3). - р.152–159.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=272340	ru_RU
dc.description.abstract	Biological Communications	ru_RU
dc.description.abstract	Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	myoblasts	ru_RU
dc.subject	bioprinting	ru_RU
dc.subject	microcarriers	ru_RU
dc.title	Isolation, culturing and 3D bioprinting equine myoblasts	ru_RU
dc.type	Статьи в российских журналах и сборниках	ru_RU