КФУ. Карточка публикации. Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus

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BACKBONE AND SIDE CHAIN NMR ASSIGNMENTS FOR THE RIBOSOME MATURATION FACTOR P (RIMP) FROM STAPHYLOCOCCUS AUREUS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2022

Язык

английский

Авторы, сотрудники КФУ

Гараева Наталия Сергеевна, автор

Библиографическое описание на языке оригинала

N. S. Garaeva Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus/ N.S.Garaeva, A. G. Bikmullin, Bruno Keiffer, Sh. Z. Validov, M.M. Yusupov, K.S. Usachev//Biomolecular NMR Assignments. - 2022. - V.16. - P.373-377.

Аннотация

The ribosomal maturation factor (RimP) is a 17.7 kDa protein and is the assembly factor of the 30S subunit. RimP is essential for efficient processing of 16S rRNA and maturation (assembly) of the 30S ribosome. It was suggested that RimP takes part in stabilization of the central pseudoknot at the early stages of the 30S subunit maturation, and this process may occur before the head domain assembly and later stages of the 30S assembly, but the mechanism of this interaction is still not fully understood. Here we report the assignment of the 1H, 13C and 15N chemical shift in the backbone and side chains of RimP from Staphylococcus aureus. Analysis of chemical shifts of the main chain using TALOS + suggests that the RimP contains eight β-strands and three α-helices with the topology α1-β1-β2-α2- β3- α3- β4- β5- β6- β7- β8. Structural studies of RimP and its complex with the ribosome by integrated structural biology approaches (NMR spectroscopy, X-ray diffraction analysis and cryoelectron microscopy) will allow further screening of highly selective inhibitors of the translation of S. aureus.

Ключевые слова

RimP, Ribosome maturation, Staphylococcus aureus, Ribosome, Protein NMR, TEV protease, Resonance assignment

Название журнала

Biomolecular NMR Assignments

URL

https://www.researchgate.net/publication/363348888_Backbone_and_side_chain_NMR_assignments_for_the_ribosome_maturation_factor_P_RimP_from_Staphylococcus_aureus

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https://repository.kpfu.ru/?p_id=272397

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Гараева Наталия Сергеевна	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	N. S. Garaeva Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus/ N.S.Garaeva, A. G. Bikmullin, Bruno Keiffer, Sh. Z. Validov, M.M. Yusupov, K.S. Usachev//Biomolecular NMR Assignments. - 2022. - V.16. - P.373-377.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=272397	ru_RU
dc.description.abstract	Biomolecular NMR Assignments	ru_RU
dc.description.abstract	The ribosomal maturation factor (RimP) is a 17.7 kDa protein and is the assembly factor of the 30S subunit. RimP is essential for efficient processing of 16S rRNA and maturation (assembly) of the 30S ribosome. It was suggested that RimP takes part in stabilization of the central pseudoknot at the early stages of the 30S subunit maturation, and this process may occur before the head domain assembly and later stages of the 30S assembly, but the mechanism of this interaction is still not fully understood. Here we report the assignment of the 1H, 13C and 15N chemical shift in the backbone and side chains of RimP from Staphylococcus aureus. Analysis of chemical shifts of the main chain using TALOS + suggests that the RimP contains eight β-strands and three α-helices with the topology α1-β1-β2-α2- β3- α3- β4- β5- β6- β7- β8. Structural studies of RimP and its complex with the ribosome by integrated structural biology approaches (NMR spectroscopy, X-ray diffraction analysis and cryoelectron microscopy) will allow further screening of highly selective inhibitors of the translation of S. aureus.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	RimP	ru_RU
dc.subject	Ribosome maturation	ru_RU
dc.subject	Staphylococcus aureus	ru_RU
dc.subject	Ribosome	ru_RU
dc.subject	Protein NMR	ru_RU
dc.subject	TEV protease	ru_RU
dc.subject	Resonance assignment	ru_RU
dc.title	Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU