| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2022 |
| Язык | русский |
|
Ситдикова Гузель Фаритовна, автор
Яковлев Алексей Валерьевич, автор
Яковлева Ольга Владиславовна, автор
|
| Библиографическое описание на языке оригинала |
A. V. Yakovlev, S. A. Dmitrieva, A. N. Krasnova, O. V. Yakovleva and G. F. Sitdikova Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia // Neurochemical Journal, 2022, Vol. 16, No. 3, pp. 263–270. |
| Аннотация |
Homocysteine is a sulfur-containing amino acid formed from methionine and considered as a risk
factor for a number of pathologies. An increase in the homocysteine level (hyperhomocysteinemia, HHcy)
during pregnancy leads to various complications of pregnancy, fetal hypoxia and, consequently, development
of early and delayed postnatal pathologies. One of the main mechanisms of homocysteine action is induction
of oxidative stress. The aim of our study was to analyze oxidative modification of proteins and activity of pro-
teases, as well as the level of oxidative stress in the brain tissue of rats with prenatal HHcy in the first week
after birth. Experimental HHcy was induced in female rats by feeding them with elevated amounts of methi-
onine for 3 weeks before, during, and after pregnancy. In the homogenates of brain tissue of offspring with
prenatal HHcy a significant increase in spontaneous protein carbonylation was observed. This result indicates
a decrease in the reserve-adaptive potential of brain cells and a decrease in the resistance of the tissue to the
action of free radicals. In the brains of rats with prenatal HHcy, the activity of acidic and neutral proteases
was higher compared to controls which could be a result of aggregation and fragmentation of protein mole-
cules due to carbonylation of amino acid residues. Brain tissues of newborn rats with prenatal HHcy were also
characterized by a high content of hydrogen peroxide, malondialdehyde as the marker of lipid peroxidation,
and a decrease in the activity of the antioxidant enzymes superoxide dismutase, catalase, glutathione perox-
idase, and glutathione reductase. Thus, the data obtained indicate a significant increase in the irreversible
process of oxidative modification of proteins in the brain of newborn rats with prenatal HHcy due to oxidative
stress. These processes contribute to the mechanisms of homocysteine neurotoxicity during the critical period
of brain development in the early postnatal period, during which intensive neurogenesis, synaptogenesis, and
formation of neural networks take place. |
| Ключевые слова |
oxidative modification of proteins, prenatal hyperhomocysteinemia, brain, proteases, antioxidant systems, lipid peroxidation |
| Название журнала |
Neurochemical Journal
|
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=276245 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Ситдикова Гузель Фаритовна |
ru_RU |
| dc.contributor.author |
Яковлев Алексей Валерьевич |
ru_RU |
| dc.contributor.author |
Яковлева Ольга Владиславовна |
ru_RU |
| dc.date.accessioned |
2022-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2022-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2022 |
ru_RU |
| dc.identifier.citation |
A. V. Yakovlev, S. A. Dmitrieva, A. N. Krasnova, O. V. Yakovleva and G. F. Sitdikova Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia // Neurochemical Journal, 2022, Vol. 16, No. 3, pp. 263–270. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=276245 |
ru_RU |
| dc.description.abstract |
Neurochemical Journal |
ru_RU |
| dc.description.abstract |
Homocysteine is a sulfur-containing amino acid formed from methionine and considered as a risk
factor for a number of pathologies. An increase in the homocysteine level (hyperhomocysteinemia, HHcy)
during pregnancy leads to various complications of pregnancy, fetal hypoxia and, consequently, development
of early and delayed postnatal pathologies. One of the main mechanisms of homocysteine action is induction
of oxidative stress. The aim of our study was to analyze oxidative modification of proteins and activity of pro-
teases, as well as the level of oxidative stress in the brain tissue of rats with prenatal HHcy in the first week
after birth. Experimental HHcy was induced in female rats by feeding them with elevated amounts of methi-
onine for 3 weeks before, during, and after pregnancy. In the homogenates of brain tissue of offspring with
prenatal HHcy a significant increase in spontaneous protein carbonylation was observed. This result indicates
a decrease in the reserve-adaptive potential of brain cells and a decrease in the resistance of the tissue to the
action of free radicals. In the brains of rats with prenatal HHcy, the activity of acidic and neutral proteases
was higher compared to controls which could be a result of aggregation and fragmentation of protein mole-
cules due to carbonylation of amino acid residues. Brain tissues of newborn rats with prenatal HHcy were also
characterized by a high content of hydrogen peroxide, malondialdehyde as the marker of lipid peroxidation,
and a decrease in the activity of the antioxidant enzymes superoxide dismutase, catalase, glutathione perox-
idase, and glutathione reductase. Thus, the data obtained indicate a significant increase in the irreversible
process of oxidative modification of proteins in the brain of newborn rats with prenatal HHcy due to oxidative
stress. These processes contribute to the mechanisms of homocysteine neurotoxicity during the critical period
of brain development in the early postnatal period, during which intensive neurogenesis, synaptogenesis, and
formation of neural networks take place. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
oxidative modification of proteins |
ru_RU |
| dc.subject |
prenatal hyperhomocysteinemia |
ru_RU |
| dc.subject |
brain |
ru_RU |
| dc.subject |
proteases |
ru_RU |
| dc.subject |
antioxidant systems |
ru_RU |
| dc.subject |
lipid peroxidation |
ru_RU |
| dc.title |
Levels of Protein Carbonylation and Activity of Proteases in the Brain
of Newborn Rats with Prenatal Hyperhomocysteinemia |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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