КФУ. Карточка публикации. Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum

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INDUCED EXPRESSION OF CYP51A AND HK1 GENES ASSOCIATED WITH PENCONAZOLE AND FLUDIOXONIL RESISTANCE IN THE POTATO PATHOGEN FUSARIUM OXYSPORUM

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2023

Язык

английский

Авторы, сотрудники КФУ

Костенникова Зарина Сирожидиновна, автор

Лутфуллин Марат Тафкилевич, автор

Лутфуллина Гузель Фанисовна, автор

Марданова Айслу Миркасымовна, автор

Другие авторы

Afordoanyi Daniel M., автор

Akosah A. Yaw, автор

Вологин Семен Германович, автор

Библиографическое описание на языке оригинала

Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257

Аннотация

Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time.

Ключевые слова

CYP51; Fusarium; Solanum tuberosum L.; dry rot; fludioxonil; histidine kinase; penconazole; wilt.

Название журнала

Microorganisms

URL

https://doi.org/10.3390/microorganisms11051257

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https://repository.kpfu.ru/?p_id=283877

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Костенникова Зарина Сирожидиновна	ru_RU
dc.contributor.author	Лутфуллин Марат Тафкилевич	ru_RU
dc.contributor.author	Лутфуллина Гузель Фанисовна	ru_RU
dc.contributor.author	Марданова Айслу Миркасымовна	ru_RU
dc.contributor.author	Afordoanyi Daniel M.	ru_RU
dc.contributor.author	Akosah A. Yaw	ru_RU
dc.contributor.author	Вологин Семен Германович	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=283877	ru_RU
dc.description.abstract	Microorganisms	ru_RU
dc.description.abstract	Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU