| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2023 |
| Язык | русский |
|
Ситдикова Гузель Фаритовна, автор
Яковлев Алексей Валерьевич, автор
|
| Библиографическое описание на языке оригинала |
A.V. Yakovlev, E. Kurmashova, E. Gataulina, E. Gerasimova, I. Khalilov, G.F. Sitdikova, Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats, Life Sciences, Volume 329, 2023, 121953, ttps://doi.org/10.1016/j.lfs.2023.121953. |
| Аннотация |
Neonatal seizures are severe pathologies which may result in long-term neurological consequences. High plasma concentrations of homocysteine – hyperhomocysteinemia (hHCy) - are associated with epilepsy. In the present study, we evaluated susceptibility to seizure of neonatal rats with prenatal hHCy.
Main methods
Prenatal hHCy was induced by feeding females with a high-methionine diet. Experiments were performed on pups during the first three postnatal weeks. Flurothyl-induced epileptic behavior was assessed according to Racine's scale. Epileptiform activity in the hippocampus was recorded using electrophysiological methods. The balance of excitation/inhibition, functional GABAergic inhibition and GABA reversal potential in hippocampal neurons were analyzed.
Key findings
Rats with hHCy developed more severe stages of behavioral patterns during flurothyl-induced epilepsy with shorter latency. Electrophysiological recordings demonstrated higher background neuronal activity in rats with hHCy. Seizure-like events triggered by flurothyl (in vivo) or 4-aminopyridine (in vitro) showed shorter latency, higher power and amplitude. An increased glutamate/GABA synaptic ratio was shown in the pyramidal neurons of rats with hHCy and more slices demonstrated excitation by isoguvacine, a selective GABA(A) receptor agonist, during the first and second postnatal weeks. The GABA driving force and the reversal potential of GABA(A) currents were more positive during the second postnatal week for hHCy rats.
Significance
The higher susceptibility to seizures in rats with prenatal hHCy due to a shift in the balance of excitation/inhibition toward excitation may underlie the clinical evidence about the association of hHCy with an increased risk of epilepsy. |
| Ключевые слова |
EpilepsyImmature ratshippocampusHomocysteineExcitation/inhibition balanceGABA driving force |
| Название журнала |
Life Sciences
|
| URL |
https://www.sciencedirect.com/science/article/pii/S002432052300588X |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=286718 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Ситдикова Гузель Фаритовна |
ru_RU |
| dc.contributor.author |
Яковлев Алексей Валерьевич |
ru_RU |
| dc.date.accessioned |
2023-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2023-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2023 |
ru_RU |
| dc.identifier.citation |
A.V. Yakovlev, E. Kurmashova, E. Gataulina, E. Gerasimova, I. Khalilov, G.F. Sitdikova, Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats, Life Sciences, Volume 329, 2023, 121953, ttps://doi.org/10.1016/j.lfs.2023.121953. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=286718 |
ru_RU |
| dc.description.abstract |
Life Sciences |
ru_RU |
| dc.description.abstract |
Neonatal seizures are severe pathologies which may result in long-term neurological consequences. High plasma concentrations of homocysteine – hyperhomocysteinemia (hHCy) - are associated with epilepsy. In the present study, we evaluated susceptibility to seizure of neonatal rats with prenatal hHCy.
Main methods
Prenatal hHCy was induced by feeding females with a high-methionine diet. Experiments were performed on pups during the first three postnatal weeks. Flurothyl-induced epileptic behavior was assessed according to Racine's scale. Epileptiform activity in the hippocampus was recorded using electrophysiological methods. The balance of excitation/inhibition, functional GABAergic inhibition and GABA reversal potential in hippocampal neurons were analyzed.
Key findings
Rats with hHCy developed more severe stages of behavioral patterns during flurothyl-induced epilepsy with shorter latency. Electrophysiological recordings demonstrated higher background neuronal activity in rats with hHCy. Seizure-like events triggered by flurothyl (in vivo) or 4-aminopyridine (in vitro) showed shorter latency, higher power and amplitude. An increased glutamate/GABA synaptic ratio was shown in the pyramidal neurons of rats with hHCy and more slices demonstrated excitation by isoguvacine, a selective GABA(A) receptor agonist, during the first and second postnatal weeks. The GABA driving force and the reversal potential of GABA(A) currents were more positive during the second postnatal week for hHCy rats.
Significance
The higher susceptibility to seizures in rats with prenatal hHCy due to a shift in the balance of excitation/inhibition toward excitation may underlie the clinical evidence about the association of hHCy with an increased risk of epilepsy. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
|
ru_RU |
| dc.title |
Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
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