КФУ. Карточка публикации. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens

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THE EFFECT OF FICIN IMMOBILIZED ON CARBOXYMETHYL CHITOSAN ON BIOFILMS OF ORAL PATHOGENS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2023

Язык

английский

Авторы, сотрудники КФУ

Байдамшина Диана Рафисовна, автор

Гафарова Ляйсан Фаридовна, автор

Зеленихин Павел Валерьевич, автор

Каюмов Айрат Рашитович, автор

Мельник Анастасия Павловна, автор

Остолоповская Ольга Вячеславовна, автор

Соколова Евгения Александровна, автор

Тризна Елена Юрьевна, автор

Библиографическое описание на языке оригинала

Baidamshina, D.R. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens / D.R. Baidamshina, E.Yu. Trizna, S.S. Goncharova, A.V. Sorokin M.S. Lavlinskaya A.P. Melnik L.F. Gafarova, M.A. Kharitonova, O.V. Ostolopovskaya, V.G. Artyukhov, E.A. Sokolova, M.G. Holyavka, M.I. Bogachev, А.R. Kayumov, P.V. Zelenikhin // International Journal of Molecular Sciences – 2023. – V.24. – N 16090. https://doi.org/10.3390/ijms242216090

Аннотация

In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19–32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius, Streptococcus gordonii, Streptococcus mutans, and Candida albicans, while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii. By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.

Ключевые слова

carboxymethyl chitosan; ficin; oral microbiota; bacterial biofilms

Название журнала

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

URL

https://www.mdpi.com/1422-0067/24/22/16090

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https://repository.kpfu.ru/?p_id=292119

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Байдамшина Диана Рафисовна	ru_RU
dc.contributor.author	Гафарова Ляйсан Фаридовна	ru_RU
dc.contributor.author	Зеленихин Павел Валерьевич	ru_RU
dc.contributor.author	Каюмов Айрат Рашитович	ru_RU
dc.contributor.author	Мельник Анастасия Павловна	ru_RU
dc.contributor.author	Остолоповская Ольга Вячеславовна	ru_RU
dc.contributor.author	Соколова Евгения Александровна	ru_RU
dc.contributor.author	Тризна Елена Юрьевна	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Baidamshina, D.R. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens / D.R. Baidamshina, E.Yu. Trizna, S.S. Goncharova, A.V. Sorokin M.S. Lavlinskaya A.P. Melnik L.F. Gafarova, M.A. Kharitonova, O.V. Ostolopovskaya, V.G. Artyukhov, E.A. Sokolova, M.G. Holyavka, M.I. Bogachev, А.R. Kayumov, P.V. Zelenikhin // International Journal of Molecular Sciences – 2023. – V.24. – N 16090. https://doi.org/10.3390/ijms242216090	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=292119	ru_RU
dc.description.abstract	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES	ru_RU
dc.description.abstract	In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19–32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius, Streptococcus gordonii, Streptococcus mutans, and Candida albicans, while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii. By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU