КФУ. Карточка публикации. Bacterial Biodiversity of the Kapova Karst Cave as a Source of HydrolasesProducers

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BACTERIAL BIODIVERSITY OF THE KAPOVA KARST CAVE AS A SOURCE OF HYDROLASESPRODUCERS

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2023

Авторы, сотрудники КФУ

Ильинская Ольга Николаевна, автор

Курди Уильям , автор

Яковлева Галина Юрьевна, автор

Авторы, аспиранты КФУ

Курди Уильям , автор

Библиографическое описание на языке оригинала

Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave (Shulgan?Tash Nature Reserve, Bashkortostan), and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate (RNA, casein, starch).

Аннотация

European Journal of Biology

Ключевые слова

Karst cave, biodiversity, cultivated bacteria, RNase, protease, amylase

Место издания

Istanbul

Название журнала

European Journal of Biology

Издательство

Istanbul university press

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https://repository.kpfu.ru/?p_id=295428

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Ильинская Ольга Николаевна	ru_RU
dc.contributor.author	Курди Уильям	ru_RU
dc.contributor.author	Яковлева Галина Юрьевна	ru_RU
dc.contributor.author	Курди Уильям	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave (Shulgan?Tash Nature Reserve, Bashkortostan), and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate (RNA, casein, starch).	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=295428	ru_RU
dc.description.abstract	European Journal of Biology	ru_RU
dc.language.iso	ru	ru_RU
dc.publisher	Istanbul university press	ru_RU
dc.subject	Karst cave	ru_RU
dc.subject	biodiversity	ru_RU
dc.subject	cultivated bacteria	ru_RU
dc.subject	RNase	ru_RU
dc.subject	protease	ru_RU
dc.subject	amylase	ru_RU
dc.title	Bacterial Biodiversity of the Kapova Karst Cave as a Source of HydrolasesProducers	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU