КФУ. Карточка публикации. Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn.

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NUCLEAR GENOME STABILITY IN LONG-TERM CULTIVATED CALLUS LINES OF FAGOPYRUM TATARICUM (L.) GAERTN.

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2017

Язык

английский

Авторы, сотрудники КФУ

Румянцева Наталья Ивановна, автор

Другие авторы

Betekhtin Alexander , автор

Rojek Magdalena , автор

Библиографическое описание на языке оригинала

Betekhtin A, Rojek M, Jaskowiak J, Milewska-Hendel A, Kwasniewska J, Kostyukova Y, et al. (2017) Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn. PLoS ONE 12(3): e0173537. https://doi.org/10.1371/journal.pone.0173537

Аннотация

Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the non-morphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2–3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines.

Ключевые слова

Fagopyrum tataricum, morphogenic callus, non-morphogenic callus, long-term cultivation, DNA content variability, nuclear genome stability

Название журнала

PLos ONE

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https://repository.kpfu.ru/?p_id=296408

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Румянцева Наталья Ивановна	ru_RU
dc.contributor.author	Betekhtin Alexander	ru_RU
dc.contributor.author	Rojek Magdalena	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Betekhtin A, Rojek M, Jaskowiak J, Milewska-Hendel A, Kwasniewska J, Kostyukova Y, et al. (2017) Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn. PLoS ONE 12(3): e0173537. https://doi.org/10.1371/journal.pone.0173537	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=296408	ru_RU
dc.description.abstract	PLos ONE	ru_RU
dc.description.abstract	Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the non-morphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2–3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Fagopyrum tataricum	ru_RU
dc.subject	morphogenic callus	ru_RU
dc.subject	non-morphogenic callus	ru_RU
dc.subject	long-term cultivation	ru_RU
dc.subject	DNA content variability	ru_RU
dc.subject	nuclear genome stability	ru_RU
dc.title	Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn.	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU