ÊÔÓ. Êàðòî÷êà ïóáëèêàöèè. Comparative analysis of the testicular and uretral microbiota in azoospermia patients using next generation sequencing (NGS)«;« [á¹ðòýøõûýžù ðýðûø÷ üøú¹þñøþž úðýø Ÿø¿úð ø ƒ¹õ¹ž ƒ ÿð³øõýþò ¸ ð÷þþ¸ÿõ¹üøõùüõþôþü ¸õúòõýø¹þòðýøŸ ýþòþóþ ÿþúþûõýøŸ (NGS)]

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COMPARATIVE ANALYSIS OF THE TESTICULAR AND URETRAL MICROBIOTA IN AZOOSPERMIA PATIENTS USING NEXT GENERATION SEQUENCING (NGS)&LAQUO;;&LAQUO; [Á¹ÐÒÝØ€ÕÛÝŽÙ ÐÝÐÛØ× ÜØÚ¹ÞÑØÞ€Ž €ÚÐÝØ Ø¯ÚÐ Ø ¹Õ€¹Ž ßÐ²ØÕÝ€ÞÒ ¨ Ð×ÞÞ¨ßÕ¹ÜØÕÙÜÕ€ÞÔÞÜ ¨ÕÚÒÕÝØ¹ÞÒÐÝØ ÝÞÒÞÓÞ ßÞÚÞÛÕÝØ (NGS)]

Ôîðìà ïðåäñòàâëåíèÿ

Ñòàòüè â çàðóáåæíûõ æóðíàëàõ è ñáîðíèêàõ

Ãîä ïóáëèêàöèè

2024

ßçûê

àíãëèéñêèé

Àâòîðû, ñîòðóäíèêè ÊÔÓ

Ãðèãîðüåâà Òàòüÿíà Âëàäèìèðîâíà, àâòîð

Ìàðêåëîâà Ìàðèÿ Èâàíîâíà, àâòîð

Õóñíóòäèíîâà Äèëÿðà Ðàøèäîâíà, àâòîð

Áèáëèîãðàôè÷åñêîå îïèñàíèå íà ÿçûêå îðèãèíàëà

Faniev M.V, Prokopiev Ya.V, Faustova K.V, Comparative analysis of the testicular and uretral microbiota in azoospermia patients using next generation sequencing (NGS)«;« [á¹ðòýøõûýžù ðýðûø÷ üøú¹þñøþž úðýø Ÿø¿úð ø ƒ¹õ¹ž ƒ ÿð³øõýþò ¸ ð÷þþ¸ÿõ¹üøõùüõþôþü ¸õúòõýø¹þòðýøŸ ýþòþóþ ÿþúþûõýøŸ (NGS)]//Opuholi Zenskoj Reproduktivnoj Sistemy. - 2024. - Vol.26, Is.2. - P.120-126.

Àííîòàöèÿ

Aim. To determine the role of taxonomic bacterial diversity of the urethral and testicular microbiota in the development of male infertility using high-throughput next-generation sequencing (NGS). Material and methods. In the period between 2018 and 2022 we examined 53 patients. Three study groups were formed: Infertile patients with nonobstructive azoospermia and concomitant varicocele (n = 13) - group 1; Infertile patients with obstructive azoospermia (n = 29) - group 2; Fertile patients with confirmed paternity and testicular pathology requiring histological verification to exclude testicular oncopathology (n = 11) - group 3. Each patient underwent TESE with testicular both and urethral tissue sampling in order to compare the bacterial landscape and control the purity of the method. The obtained material was sequenced using a high-throughput method (NGS). Results. According to our findings, the testicular tissue of obstructive azoospermia patients had significantly depleted bacterial diversity in comparison to nonobstructive azoospermia and concomitant varicocele ones. Also, the fertile group turned out to be the most diverse in its taxonomic community. These results may suggest the bacterial microbiome's influence on men's reproductive health. Discussion. Our urogenital bacterial diversity analysis showed that human testicular tissue is not a microbiologically sterile environment and also presented new data associated with testicular tissue and its possible relations with male infertility. Conclusion. A focus on the qualitative and quantitative composition of the testicular microbial community may form the new basis in the diagnosis and treatment of male infertility associated with various types of azoospermia.

Êëþ÷åâûå ñëîâà

azoospermiagenomemale infertilitymicrobiomemicrobiotasequencingtesticular tissue

Íàçâàíèå æóðíàëà

Opuholi Zenskoj Reproduktivnoj Sistemy

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199010568&doi=10.62968%2f2070-9781-2024-25-2-120-126&partnerID=40&md5=2c0c7a4499efd666cdf4dd2096e84281

Ïîæàëóéñòà, èñïîëüçóéòå ýòîò èäåíòèôèêàòîð, ÷òîáû öèòèðîâàòü èëè ññûëàòüñÿ íà ýòó êàðòî÷êó

https://repository.kpfu.ru/?p_id=302710

Ïîëíàÿ çàïèñü ìåòàäàííûõ

Ïîëå DC	Çíà÷åíèå	ßçûê
dc.contributor.author	Ãðèãîðüåâà Òàòüÿíà Âëàäèìèðîâíà	ru_RU
dc.contributor.author	Ìàðêåëîâà Ìàðèÿ Èâàíîâíà	ru_RU
dc.contributor.author	Õóñíóòäèíîâà Äèëÿðà Ðàøèäîâíà	ru_RU
dc.date.accessioned	2024-01-01T00:00:00Z	ru_RU
dc.date.available	2024-01-01T00:00:00Z	ru_RU
dc.date.issued	2024	ru_RU
dc.identifier.citation	Faniev M.V, Prokopiev Ya.V, Faustova K.V, Comparative analysis of the testicular and uretral microbiota in azoospermia patients using next generation sequencing (NGS)«;« [á¹ðòýøõûýžù ðýðûø÷ üøú¹þñøþž úðýø Ÿø¿úð ø ƒ¹õ¹ž ƒ ÿð³øõýþò ¸ ð÷þþ¸ÿõ¹üøõùüõþôþü ¸õúòõýø¹þòðýøŸ ýþòþóþ ÿþúþûõýøŸ (NGS)]//Opuholi Zenskoj Reproduktivnoj Sistemy. - 2024. - Vol.26, Is.2. - P.120-126.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=302710	ru_RU
dc.description.abstract	Opuholi Zenskoj Reproduktivnoj Sistemy	ru_RU
dc.description.abstract	Aim. To determine the role of taxonomic bacterial diversity of the urethral and testicular microbiota in the development of male infertility using high-throughput next-generation sequencing (NGS). Material and methods. In the period between 2018 and 2022 we examined 53 patients. Three study groups were formed: Infertile patients with nonobstructive azoospermia and concomitant varicocele (n = 13) - group 1; Infertile patients with obstructive azoospermia (n = 29) - group 2; Fertile patients with confirmed paternity and testicular pathology requiring histological verification to exclude testicular oncopathology (n = 11) - group 3. Each patient underwent TESE with testicular both and urethral tissue sampling in order to compare the bacterial landscape and control the purity of the method. The obtained material was sequenced using a high-throughput method (NGS). Results. According to our findings, the testicular tissue of obstructive azoospermia patients had significantly depleted bacterial diversity in comparison to nonobstructive azoospermia and concomitant varicocele ones. Also, the fertile group turned out to be the most diverse in its taxonomic community. These results may suggest the bacterial microbiome's influence on men's reproductive health. Discussion. Our urogenital bacterial diversity analysis showed that human testicular tissue is not a microbiologically sterile environment and also presented new data associated with testicular tissue and its possible relations with male infertility. Conclusion. A focus on the qualitative and quantitative composition of the testicular microbial community may form the new basis in the diagnosis and treatment of male infertility associated with various types of azoospermia.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Comparative analysis of the testicular and uretral microbiota in azoospermia patients using next generation sequencing (NGS)«;« [á¹ðòýøõûýžù ðýðûø÷ üøú¹þñøþž úðýø Ÿø¿úð ø ƒ¹õ¹ž ƒ ÿð³øõýþò ¸ ð÷þþ¸ÿõ¹üøõùüõþôþü ¸õúòõýø¹þòðýøŸ ýþòþóþ ÿþúþûõýøŸ (NGS)]	ru_RU
dc.type	Ñòàòüè â çàðóáåæíûõ æóðíàëàõ è ñáîðíèêàõ	ru_RU