КФУ. Карточка публикации. ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ mTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ

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ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ MTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ

Форма представления

Статьи в российских журналах и сборниках

Год публикации

2025

Язык

русский

Авторы, сотрудники КФУ

Абрамова Зинаида Ивановна, автор

Другие авторы

Афанасьева Зинаида Александровна, автор

Авторы, студенты КФУ

Бурцева Анастасия Владимировна, автор

Тихонова Анастасия Николаевна, автор

Библиографическое описание на языке оригинала

Бурцева А.В. Влияние окислительного стресса на активность факторов апоптоза и аутофагии связанных с митохондриями CD4+Т лимфоцитов при аутоиммунном тиреоидите / А.В Бурцева, А.Н.Тихонова, З. А. Афанасьева, З.И. Абрамова // Медицинская иммунология.-2025.-Т.27,№ 4.-С.847-862

Аннотация

Current studies of autoimmunity processes are addressing specific processes involving mitochondria, i.e., altered intracellular signaling including the generation of ROS and release of proteins from mitochondria to the cytoplasm, thus activating apoptosis. These functions of mitochondria are usually associated with disturbed bioenergetic functions and excessive production of H2O2. Currently, a new area is actively developing, i.e., potential participation of mitochondria in the immune response. Therefore, the aim of the present study was to evaluate the dynamics of apoptosis factors and autophagy associated with mitochondria of CD4+T cells in autoimmune thyroiditis (AIT). The study was performed with CD4+T cells of AIT patients and healthy donors obtained by magnetic separation. Apoptosis and autophagy were assessed by flow cytometry. Western blotting was used to analyze autophagy marker proteins (p62, LC3I/II, mTOR kinase), and apo ptosis regulator Bcl- 2 in CD4+T lymphocytes of the patients and donors. The state of mitochondria in CD4+T lymphocytes was assessed by confocal microscopy. Using an experimental model with increased H2O2 , we showed that ROS activate LC3 protein in cells of patients with AIT along with accumulation of autophagic adapter protein p62, as registered on the outer mitochondrial membrane. An increased level of mitophagoses was found in CD4+T cells from the AIT patients. On the basis of these data, one may assume that H2O2 causes activation of mitophagy in CD4+T cells of patients with AIT, and the development of oxidative stress with excessive production of ROS leads to irreversible damage to mitochondria, which causes a decreased apoptotic activity followed by development of secondary necrosis of CD4+T lymphocytes in AIT, making them cytotoxic. Accumulation of such cells in the thyroid tissue may lead to impaired apoptosis in thyrocytes and, as a consequence, to secondary necrosis, thus resulting in development of autoimmune response. The obtained data require additional studies, since the detection of CD4+T lymphocytes prone for apoptosis or necrosis in patients with AIT may be used as a diagnostic criterion for prediction of inflammatory conditions.

Ключевые слова

окислительный стресс, mTOR, CD4+Т-лимфоцит, митофагия, аутоиммунном тиреоидит

Название журнала

Медицинская иммунология

URL

https://doi.org/10.15789/1563-0625-IOO-3141

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https://repository.kpfu.ru/?p_id=312467

Файлы ресурса

Название файла	Размер (Мб)	Формат
F_VLIYaNIE_OKISLITELNOGO_STRESSA_NA_AKTIVNOST..._AIT.pdf	0,45	pdf	посмотреть / скачать

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Абрамова Зинаида Ивановна	ru_RU
dc.contributor.author	Афанасьева Зинаида Александровна	ru_RU
dc.contributor.author	Бурцева Анастасия Владимировна	ru_RU
dc.contributor.author	Тихонова Анастасия Николаевна	ru_RU
dc.date.accessioned	2025-01-01T00:00:00Z	ru_RU
dc.date.available	2025-01-01T00:00:00Z	ru_RU
dc.date.issued	2025	ru_RU
dc.identifier.citation	Бурцева А.В. Влияние окислительного стресса на активность факторов апоптоза и аутофагии связанных с митохондриями CD4+Т лимфоцитов при аутоиммунном тиреоидите / А.В Бурцева, А.Н.Тихонова, З. А. Афанасьева, З.И. Абрамова // Медицинская иммунология.-2025.-Т.27,№ 4.-С.847-862	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=312467	ru_RU
dc.description.abstract	Медицинская иммунология	ru_RU
dc.description.abstract	Current studies of autoimmunity processes are addressing specific processes involving mitochondria, i.e., altered intracellular signaling including the generation of ROS and release of proteins from mitochondria to the cytoplasm, thus activating apoptosis. These functions of mitochondria are usually associated with disturbed bioenergetic functions and excessive production of H2O2. Currently, a new area is actively developing, i.e., potential participation of mitochondria in the immune response. Therefore, the aim of the present study was to evaluate the dynamics of apoptosis factors and autophagy associated with mitochondria of CD4+T cells in autoimmune thyroiditis (AIT). The study was performed with CD4+T cells of AIT patients and healthy donors obtained by magnetic separation. Apoptosis and autophagy were assessed by flow cytometry. Western blotting was used to analyze autophagy marker proteins (p62, LC3I/II, mTOR kinase), and apo ptosis regulator Bcl- 2 in CD4+T lymphocytes of the patients and donors. The state of mitochondria in CD4+T lymphocytes was assessed by confocal microscopy. Using an experimental model with increased H2O2 , we showed that ROS activate LC3 protein in cells of patients with AIT along with accumulation of autophagic adapter protein p62, as registered on the outer mitochondrial membrane. An increased level of mitophagoses was found in CD4+T cells from the AIT patients. On the basis of these data, one may assume that H2O2 causes activation of mitophagy in CD4+T cells of patients with AIT, and the development of oxidative stress with excessive production of ROS leads to irreversible damage to mitochondria, which causes a decreased apoptotic activity followed by development of secondary necrosis of CD4+T lymphocytes in AIT, making them cytotoxic. Accumulation of such cells in the thyroid tissue may lead to impaired apoptosis in thyrocytes and, as a consequence, to secondary necrosis, thus resulting in development of autoimmune response. The obtained data require additional studies, since the detection of CD4+T lymphocytes prone for apoptosis or necrosis in patients with AIT may be used as a diagnostic criterion for prediction of inflammatory conditions.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	окислительный стресс	ru_RU
dc.subject	mTOR	ru_RU
dc.subject	CD4+Т-лимфоцит	ru_RU
dc.subject	митофагия	ru_RU
dc.subject	аутоиммунном тиреоидит	ru_RU
dc.title	ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ mTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ	ru_RU
dc.type	Статьи в российских журналах и сборниках	ru_RU